Crystal Structure of S-adenosyl-L-homocysteine hydrolase from Acanthamoeba castellanii with bound NAD and Adenosine

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

3-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

MAHHHHHHMQTQTQTQPAGNAFQYEIRDISLAEFGRKEINLAEHEMPGLMQTREKYGAEQPLKGVRLAGSLHMTIQTAVL
IETLQALGANVRWCSCNIFSTQDHAAAAIVAAGTPVFAWKGETLEEYWECTWKTLLFPDDMGPQLIVDDGGDATLMVHRG
FYAEDNPSILDDDEGSEELAIVNKLLKRIQKEKPGYWHKIVPELKGVSEETTTGVHRLYEMMKEGKLLFPALNVNDSVTK
SKFDNVYGCRHSLVDAIMRATDVMLSGKVACVLGYGDVGKGSAESLKGQGARVVVTEVDPICALQACMAGYEVVRIEDVL
DKAEIFVTTTGNCDIIRIEHMEKMRHNAIVCNIGHFDNEIQVKALKEFPGIKRIEIKPQVDQFVFPDGHAIVLLAEGRLV
NLGCATGHPSFVMSNSFTNQTLAQISLWKEKYELGVYTLPKKLDEEVARLHLEKLGAKLTVLTDKQAKYLGIAKDGPYKP
DHYRY

AccaA.00032.a.B1.PW38645 at 20.81 mg/ml, incubated with 2 mM NAD, SAH, mixed 1:1 with 12.5% (w/v) PEG-1000, 12.5% (w/v) PEG-3350, 12.5% (v/v) MPD, 0.1 M MOPS/HEPES-Na, pH=7.5, 0.02 M of sodium formate, ammonium acetate, trisodium citrate, sodium potassium L-tartrate, 0.2 M sodium oxamate. Tray: 311079g8, puck: ved0-8

Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases

SSGCID, GS-adenosyl-L-homocysteine hydrolase, NAD, AMP, Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, HYDROLASE

MQTQTQTQPAGNAFQYEIRDISLAEFGRKEINLAEHEMPGLMQTREKYGAEQPLKGVRLAGSLHMTIQTAVLIETLQALG
ANVRWCSCNIFSTQDHAAAAIVAAGTPVFAWKGETLEEYWECTWKTLLFPDDMGPQLIVDDGGDATLMVHRGFYAEDNPS
ILDDDEGSEELAIVNKLLKRIQKEKPGYWHKIVPELKGVSEETTTGVHRLYEMMKEGKLLFPALNVNDSVTKSKFDNVYG
CRHSLVDAIMRATDVMLSGKVACVLGYGDVGKGSAESLKGQGARVVVTEVDPICALQACMAGYEVVRIEDVLDKAEIFVT
TTGNCDIIRIEHMEKMRHNAIVCNIGHFDNEIQVKALKEFPGIKRIEIKPQVDQFVFPDGHAIVLLAEGRLVNLGCATGH
PSFVMSNSFTNQTLAQISLWKEKYELGVYTLPKKLDEEVARLHLEKLGAKLTVLTDKQAKYLGIAKDGPYKPDHYRY