Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions

(2S)-2-amino-4-(7-hydroxy-2-oxo-2H-1-benzopyran-4-yl)butanoic acid

Structural Origins of Altered Spectroscopic Properties upon Ligand Binding in Proteins Containing a Fluorescent Noncanonical Amino Acid.

MAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATT
WSGQYVGGAEARINTQWLLT(DV7)GTTEANAWKSTLVGHDTFTKVKPSAASLEHHHHHH

MAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATT
WSGQYVGGAEARINTQWLLTXGTTEANAWKSTLVGHDTFTKVKPSAASLEHHHHHH

500 ul of 0.1 M Citric Acid, pH 3.5, 3M NaCl in reservoir with 2 ul of reservoir buffer mixed with 2 ul of 10 mg/ml of protein in the drop. Crystals were soaked to pH 5.5 overnight with 3 exchanges

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

unnatural amino acid, non-canonical amino acid, fluorescence, biosensor, small molecule biosensor, ligand detection, BIOTIN BINDING PROTEIN

AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTW
SGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS

binding site for Ligand residues DV7 A 112 through GLY A 113 bound to THR A 111

binding site for Ligand residues DV7 B 112 through GLY B 113 bound to THR B 111