Isolated S3D-cofilin bound to an actin filament

Structures of cofilin-induced structural changes reveal local and asymmetric perturbations of actin filaments.

Segments with isolated, bound S3D-cofilin were split into even and odd halves for FSC calculations.

Complex of isolated human S3D-cofilin-1 bound to rabbit skeletal actin filaments

Both bare and S3D-cofilin-decorated filament segments were selected and initially refined together.

18 kDa phosphoprotein,p18,Cofilin,non-muscle isoform

MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITN
WDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG
VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEIT
ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

MADGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDC
RYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVIS
LEGKPL

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

WT Cofilactin from partially cofilin-decorated actin filaments

Bare actin from partially WT cofilin-decorated actin filaments

Isolated, bound WT cofilin on actin filaments

Barbed boundary of a WT cofilin cluster bound to actin filaments

The molecules in the experiments were: Rabbit actin (UniProt P68135) and Human cofilin-1 (UniProt P23528), however the sequences modeled in the coordinates correspond to the sequences from Chicken actin and Chicken cofilin-2 since those were pdb models of homolog molecules used to fit into the cryo-EM density maps.

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDC
RYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVIS
LEGKPL