Structure of E70A mutant of Rex8A from Paenibacillus barcinonensis complexed with 2(3)-alpha-L-arabinofuranosyl-xylotriose.

alpha-L-arabinose; L-arabinose; arabinose

Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.

Reducing-end xylose-releasing exo-oligoxylanase Rex8A

MNITGKGAYDTGTYANLFQRSGYREDEIKARLEQTWNDLFYGDEHTRIYYPVGDDKGYMLDTGNDDVRSAGMSYGMMMAV
QMDKKHEFDRLWNYAYTYMQHTEGRYKDYFAWHCKPDGTRLSPGPAPDGEEFFAMALFFASNRWGDGPAPYDYQAQARKI
LHACLHQGEQGEGDPMWEPSNRLIKFIPELPFSDPSYHLPHFYELFAQYANEQDRTFWKEAAEASRAYLRTACHPVTGLS
PEYANYDGTPAPVQLHGDFRHFYSDAYRVAANVALDWEWFRKDPWQVQQSNRIQAFFSDIDVSDYRRYTIEGEPFNEPAL
HPVGLLATNAMASLAADGPDADSFVKRFWNTPLRQGKRRYYDNCLYFFTMLALSGNYRVYQQAAA

18% PEG 3350, 0.2M Potassium thiocyanate, 0.1M Bis-Tris propane pH 7.5. Microseeding. Co-crystallization with 2(3)-alpha-L-arabinofuranosyl-xylotriose

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

Xylan, Exo-oligoxylanase, Xylanases, Xylooligosaccharides, Xylooligomers, Xylose, Hydrolysis, Polysaccharides, Glycan, Glycan degradation, Xylan degradation, Glycosidase, Hydrolase, Carbohydrate metabolism.

MNITGKGAYDTGTYANLFQRSGYREDEIKARLEQTWNDLFYGDEHTRIYYPVGDDKGYMLDTGNDDVRSEGMSYGMMMAV
QMDKKHEFDRLWNYAYTYMQHTEGRYKDYFAWHCKPDGTRLSPGPAPDGEEFFAMALFFASNRWGDGPAPYDYQAQARKI
LHACLHQGEQGEGDPMWEPSNRLIKFIPELPFSDPSYHLPHFYELFAQYANEQDRTFWKEAAEASRAYLRTACHPVTGLS
PEYANYDGTPAPVQLHGDFRHFYSDAYRVAANVALDWEWFRKDPWQVQQSNRIQAFFSDIDVSDYRRYTIEGEPFNEPAL
HPVGLLATNAMASLAADGPDADSFVKRFWNTPLRQGKRRYYDNCLYFFTMLALSGNYRVY