Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk model

ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.

Mitochondrial ATP synthase dimer, peripheral stalk

Oligomycin sensitivity conferring protein (OSCP)

MTIHSCLARRAVSVAASGARAFASGLGARAVAVGALQSARLLHTSSLRAAGAKISPSEMSRLLEERIAGWKTQTSTEEVG
RVVSVGDGIARLFGLEGVQAGELVEFQNGMTGMALNLETDNVGVVIFGDDRSVLEGDSVKRTGRIVDVPIGPGLLGRVVD
ALGNPIDGKGPIPAKERRRVELKAPGIIPRKSVHEPMMTGLKCVDALVPVGRGQRELIIGDRQTGKTAVAVDAIINQKEI
NDSTDDESKKLYCIYVAVGQKRSTVAQIVKALEQRDAMKYTTVVAATASEAAPLQFLAPYSGCAMGEWFRDSGRHCVIIY
DDLSKQATAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMGDKSGGGSLTALPVIETQAGDVSAYIPTNVISITDG
QIFLETELFYKGIRPAINVGLSVSRVGSAAQVKAMKQVAGTMKLELAQYREVAAFAQFGSDLDASTRQLLTRGTALTELL
KQRQYSPMKNSVQVCVLYCGVKGYLDPLDPKEISRFESLFIDYINANHQDILKTIETEKELSEKTEAKLRAAVDEFVAMN
EFKKK

MALPLLASRRLFSSFVFRGQPSTLSSNLSLVRIRGLHGGSLSPPSATLPRAVQLFSSRIAFSTAAAEDSGASQTLEGRYA
SALFRVAKKKNQLEKVYGDLESVRNALKDSSEFRLFVDSPAVSVQQKLDVLRQLVNRYKFDPLTGNLLTTLVENKRLPML
ARVADAFDAMYRKEKGEVKCLVTSAKPLSAQQQKEIVAALQNRAGTQARLIIDYAVSPQIMGGLVVRLGEQVLDFSVATR
LDRLQSQLLAPL

MQALRRGAAIPSRLLPRRDSWMSLAPFVAPNNAAAWRKLRDGAQEVQTVIERQSTPGKPQQIDWAKWESQIAHKDILNCL
KTFYTNQVQILDRALGALETAKTPAPCEGAEKGWALFDAALSACAKSVEKSEELLSNGARALWVSCSNPPVWKVNTNEWL
DSDQYWQAFVEKHHFYSQYQPGVVDPEAPQEVEAFKQAWHSRMGKFNDRSDTPMLYAYMNELPSWEYYDLHRSAFLEHMT
YFLVRTGGDFRFFPEMPPWQWLAHMENLRFKLLSVAQSRRSQLQLANLERERALDFLPVDVEHHGEEYTQKFLQYETELF
QACAARLMGHFMFLCDPFIPVQSAEALSAVTRVDNGKGKLFSLGDDVNALFYLPEQQRRDVERPTQAVQTLLGHLEATGR
PFNPCYSELLHVHAEVLEERGEHWLTAPGECVSQAFLRRLRTDDPAYEVYCSYFKEMYERFAGAKEVSMEDGRKRLATIE
KNAQEEAAAYGLALKTMGSAELAHKAREGAAKLEQLRKAQEKAAGKSAQTVQENKM

MLNFIPKRCPSVSLLFGKRPVQRIEVGQARHQLEIPVETIEKIYEGVDSRLEYHNKDYNAMKWKDFMKLKLDAYHLLEAS
QSETAAKSALSDLNWFSDLADIYSGQQTMAEMDVALKAQGEQKLSYPIQGKNIK

MNFSSSARWLAVRQSQTLGHTTRATVAAGRRVLAHSPAATEFTSFQSLHIGGDVCKLPLAVALGAAPSALGYGSAKHNQQ
RQYATLGSGWSFSKVQYTKYRITKPWTTDTTFDDIILSQPSKEDFAKFTKEAPLFLRFLKLVTDVEGRQEAFIQFAKRCE
NGLTVEKDVYVTKKELVDCLWKNGYTDTEINAFEIAFPADYKFHYPELAVLFDLTEEDCYKYCIRQRAATPEELVELKYT
KPKNLVSSYGLCFLGVWFGLSNTVLSNAWFYSKTFPFGAVFYMLGSYFYRDIREKLWKEEKSLIHTAQENKNMGEESVYK
QMKKYATDTKCLDYLSTFRTEVEDQIANYKVALVSQMRRQLTERLVEKLNGIQQAEKLIQGSLQDVMIREIVSSFKDLYK
SRPELHDAAMQSAIQGLSGSDGAMDPVGAHFKASLQELAKVNLSTATADPMGTVVQRVAAVFQKREKEFLDTFTVKATEA
QEIKTIVDKCHKGNTFDFHALSDEELRRLEQLYSTVNNRVGFETIHENSIKPVAPLSENSKGFVEFVNTQLEITKAKLRN
ARLTAFAHAFV

Toxoplasma gondii (strain ATCC 50853 / GT1)

mitochondrial, ATP synthase, peripheral stalk, OSCP, MEMBRANE PROTEIN

MTIHSCLARRAVSVAASGARAFASGLGARAVAVGALQSARLLHTSSLRAAGAKISPSEMSRLLEERIAGWKTQTSTEEVG
RVVSVGDGIARLFGLEGVQAGELVEFQNGMTGMALNLETDNVGVVIFGDDRSVLEGDSVKRTGRIVDVPIGPGLLGRVVD
ALGNPIDGKGPIPAKERRRVELKAPGIIPRKSVHEPMMTGLKCVDALVPVGRGQRELIIGDRQTGKTAVAVDAIINQKEI
NDSTDDESKKLYCIYVAVGQKRSTVAQIVKALEQRDAMKYTTVVAATASEAAPLQFLAPYSGCAMGEWFRDSGRHCVIIY
DDLSKQATAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMGDKSGGGSLTALPVIETQAGDVSAYIPTNVISITDG
QIFLETELFYKGIRPAINVGLSVSRVGSAAQVKAMKQVAGTMKLELAQYREVAAFAQFGSDLDASTRQLLTRGTALTELL
KQRQYSPMKNSVQVCVLYCGVKGYLDPLDPKEISRFESLFIDYINANHQDILKTIETE

MQALRRGAAIPSRLLPRRDSWMSLAPFVAPNNAAAWRKLRDGAQEVQTVIERQSTPGKPQQIDWAKWESQIAHKDILNCL
KTFYTNQVQILDRALGALETAKTPAPCEGAEKGWALFDAALSACAKSVEKSEELLSNGARALWVSCSNPPVWKVNTNEWL
DSDQYWQAFVEKHHFYSQYQPGVVDPEAPQEVEAFKQAWHSRMGKFNDRSDTPMLYAYMNELPSWEYYDLHRSAFLEHMT
YFLVRTGGDFRFFPEMPPWQWLAHMENLRFKLLSVAQSRRSQLQLANLERERALDFLPVDVEHHGEEYTQKFLQYETELF
QACAARLMGHFMFLCDPFIPVQSAEALSAVARVDNGKGKLFSLGDDVNALFYLPEQQRRDVERPTQAVQTLLGHLEATGR
PFNPCYSELLHVHAEVLEERGEHWLTAPGECVSQAFLRRLRTDDPAYEVYCSYFKEMYERFAGAKEVSMEDGRKRLATIE
KNAQEEAAAYGLALKTMGSAELAHKAREGAAKLEQLRKAQEKAAGKSAQTVQENKM

MNFSSSARWLAVRQSQTLGHTTRATVAAGRRVLAHSPAATEFTSFQSSHIGGDVCKLPLAVALGAAPSALGYGSAKHNQQ
RQYATLGSGWSFSKVQYTKYRITKPWTTDTTFDDIILSQPSKEDFAKFTKEAPLFLRFLKLVTDVEGRQEAFIQFAKRCE
NGLTVEKDVYVTKKELVDCLWKNGYTDTEINAFEIAFPADYKFHYPELAVLFDLTEEDCYKYCIRQRAATPEELVELKYT
KPKNLVSSYGLCFLGVWFGLSNTVLSNAWFYSKTFPFGAVFYMLGSYFYRDIREKLWKEEKSLIHTAQENKNMGEESVYK
QMKKYATDTKCLDYLSTFRTEVEDQIANYKVALVSQMRRQLTERLVEKLNGIQQAEKLIQGSLQDVMIREIVSSFKDLYK
SRPELHDAAMQSAIQGLSGSDGAMDPVGAHFKASLQELAKVNLSTATADPMGTVVQRVAAVFQKREKEFLDAFTVKATEA
QEIKTIVDKCHKGNTFDFHALSDEELRRLEQLYSTVNNRVGFETIHENSIKPVAPLSENSKGFVEFVNTQLEITKAKLRN
ARLTAFAHAFV