Crystal structure of human Aldehyde dehydrogenase 1A3 in complex with a selective inhibitor

methyl 5-(1,3-benzodioxol-5-yl)-2-phenyl-pyrazolo[1,5-a]pyrimidine-7-carboxylate

A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis in glioma stem cells.

Aldehyde dehydrogenase family 1 member A3

Aldehyde dehydrogenase 6,Retinaldehyde dehydrogenase 3,RalDH3

MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQV
AFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDD
NVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTV
GAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRV
FVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSE
VTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGF
KMSGNGRELGEYALAEYTEVKTVTIKLGDKNP

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

20% PEG-3350
0.24 M sodium malonate pH 7.0
10 mM TCEP-hydrochloride

ALDH1A3, aldehyde dehydrogenase ALDH inhibitor Cancer stem cell, glioblastoma mesenchymal stem cell stem cell, OXIDOREDUCTASE

binding site for Di-peptide NAD D 601 and LYS D 204