Structure of a beta galactosidase with inhibitor

Mechanistic Insights into the Chaperoning of Human Lysosomal-Galactosidase Activity: Highly Functionalized Aminocyclopentanes and C -5a-Substituted Derivatives of 4- epi -Isofagomine.

Beta-galactosidase without predicted signal sequence and with N-terminal his tag

MGSSHHHHHHAAPLPELLSNNGKHALMVDGAPYIILGSQTNNSSNYPDALKDVWPSMEKMGANTLSIPVAWEQIEPVEGQ
FDFSFVDVLLKEARQRKVRLVLLWFATWKNNAPHYAPAWVKLDNARFPRVVKEDGDTLNSLSPLGQNTLAADKKAFVELM
KYLAKRDKDHTVIMVQVQNEVGTYGAVRDYSPMAQAVFNAAVPDDLIQKLQLKPGTWSQVFGRDADEFFHAYQIARYCDE
VTVAGKAIKNLPMYVNVALRNPFNPGLPGQYSSGGGTDNVLHIWKAAAPNIDLIAPDIYFRDYKTVSKVLELYTRPDNAL
FVAEIGNDQPFARYLFPTLGKGGIGFSPFGMDDTDYTNYPLGAKVYNDETIEQFAQVYRLVNPMMREWARLSYQGQVWGV
AEPLDSTTETQKIWNAEATPEEKEQHKKDRASALTQQLDLGLWDAEVTYGRPMFWVTPPEGNTPAAGGALIAQLDDNEYL
VTAYKARVEFKPSQELAGKKFMIERVEEGRFEKGKWVMERVWNGDQTDWGLNFTDRPHLLRVKMASYSVQ

Biotechnology and Biological Sciences Research Council

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY SOME ATOMS OF THE LIGAND, PARTICULARLY THE (DIMETHYLAMINO)NAPHTHALENE GROUP, IN THE ACTIVE SITES OF MOLECULES A,B,C AND H ARE NOT MODELLED. IN MOLECULES D, E, F AND G SOME LIGAND ATOMS ARE MODELLED AT PARTIAL OCCUPANCY. THERE IS SOME UNMODELLED DENSITY BETWEEN THE SIDE CHAIN OF TRP480 AND THE LIGAND SULFONYL GROUP IN MOST MOLECULES. IN SOME OF THE MOLECULES THE LOOPS BETWEEN THR433 AND LYS448 HAVE BEEN MODELLED AT REDUCED OCCUPANCY OR WITH CHAIN BREAKS WHERE RESIDUES COULD NOT BE PLACED.
beta galactosidase, inhibitor, HYDROLASE

AAPLPELLSNNGKHALMVDGAPYIILGSQTNNSSNYPDALKDVWPSMEKMGANTLSIPVAWEQIEPVEGQFDFSFVDVLL
KEARQRKVRLVLLWFATWKNNAPHYAPAWVKLDNARFPRVVKEDGDTLNSLSPLGQNTLAADKKAFVELMKYLAKRDKDH
TVIMVQVQNEVGTYGAVRDYSPMAQAVFNAAVPDDLIQKLQLKPGTWSQVFGRDADEFFHAYQIARYCDEVTVAGKAIKN
LPMYVNVALRNPFNPGLPGQYSSGGGTDNVLHIWKAAAPNIDLIAPDIYFRDYKTVSKVLELYTRPDNALFVAEIGNDQP
FARYLFPTLGKGGIGFSPFGMDDTDYTNYPLGAKVYNDETIEQFAQVYRLVNPMMREWARLSYQGQVWGVAEPLDSTTET
QKIWNAEATPEEKEQHKKDRASALTQQLDLGLWDAEVTYGRPMFWVTPPEGNTPAAGGALIAQLDDNEYLVTAYKARVEF
KPSQELAGKKFMIERVEEGRFEKGKWVMERVWNGDQTDWGLNFTDRPHLLRVKMASYSVQ