Streptavidin variants harbouring an artificial organocatalyst based cofactor

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

An Artificial Cofactor Catalyzing the Baylis-Hillman Reaction with Designed Streptavidin as Protein Host*.

MASMTGGQQMGRDEAGITGTWYNQLGSTFIVTAGADGALTGTYESAAGKAESRYVLTGRYDSAPATDGSGTALGWTVAWK
NNYRNAHSATTWSGQYVGGAEARINTQWLLTMGATEANGWASTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ

1.6M Na2HPO4 0.4M K2HPO4 0.1M Sodium citrate phosphate pH 4.2

artificial cofactor, streptavidin, catalyst, Biotin-binding protein

EAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWS
GQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ