Crystal structure of monooxygenase RutA complexed with Uracil under atmospheric pressure.

Aminoperoxide adducts expand the catalytic repertoire of flavin monooxygenases.

MMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTS
RIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGK
SDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYV
LFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVA
SVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA

100 mM Bis-Tris pH 6.5, 1900 mM ammonium sulphate, 2-5% MPD (v/v),1 mM FMN

Hydrogens have been added in their riding positions

monooxygenase, RutA, FMN, flavin-N5-oxide, bioengineering, FLAVOPROTEIN