Crystal structure of the engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL)

Engineering the protein dynamics of an ancestral luciferase.

Engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL)

TATGDEWWAKCKQVDVLDSEMSYYDSDPGKHKNTVIFLHGNPTSSYLWRNVIPHVEPLARCLAPDLIGMGKSGKLPNHSY
RFVDHYRYLSAWFDSVNLPEKVTIVCHDWGSGLGFHWCNEHRDRVKGIVHMESVVSPLKGWESFPETARDILPQALRSEA
GEEMVLKKNFFIERLLPSSIMRKLSEEEMDAYREPFVEPGESRRPTLTWPREIPIKGDGPEDVIEIVKSYNKWLSTSKDI
PKLFINADPGFFSNAIKKVTKNWPNQKTVTVKGLHFLQEDSPEEIGEAIADFLNELTK

PEG 500MME, PEG 20000, sodium nitrate, sodium phosphate dibasic, ammonium sulphate

engineered ancestral enzyme, bioluminscence, coelenterazine-utilizing enzyme, LUMINESCENT PROTEIN