Crystal structure of helicase Pif1 from Thermus oshimai in apo form

Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.

TPEGLSSEQQRAFLAVTQTPHPAHLITGPAGTGKTTLLYALQEFYKGRAVTLAPTGTAALQARGQTVHSFFRFPARLLRY
RHPEDIRPPGPHSPLRKAIEQMEVLILDEVGMVRVDLLEAMDWALRKTRKRLEEPFGGVKVLLLGDTRQLEPVVPGGEEA
LYIARTWGGPFFFQAHVWEEVALRVHRLWESQRQREDPLFAELLKRLRQGDPQALETLNRAAVRPDGGEEPGTLILTPRR
KEADALNLKRLEALPGKPLEYQAQVKGEFAETDFPTEAALTLKKGAQVILLRNDPLGEYFNGDLGWVEDLEAEALAVRLK
RNGRRVVIRPFVWEKIVYTYDSEREEIKPQVVGTFRQVPVRLAWALTVHKAQGLTLDKVHLELGRGLFAHGQLYVALTRV
RRLQDLSLSRPIAPTELLWRPEVEVFETRIQEGIWQKSHGWPSL

Sodium Hepes-MOPS 0.1M
Ethylene glycol 1.25%
PEG 4000 20%
Glycerol 20%

National Natural Science Foundation of China

APEGLSSEQQRAFLAVTQTPHPAHLITGPAGTGKTTLLYALQEFYKGRAVTLAPTGTAALQARGQTVHSFFRFPARLLRY
RHPEDIRPPGPHSPLRKAMEQMEVLILDEVGMVRVDLLEAMDWALRKTRKRLEEPFGGVKVLLLGDTRQLEPVVPGGEEA
LYIARTWGGPFFFQAHVWEEVALRVHRLWESQRQREDPLFAELLKRLRQGDPQALETLNRAAVRPDGGEEPGTLILTPRR
KEADALNLKRLEALPGKPLEYQAQVKGEFAETDFPTEAALTLKKGAQVILLRNDPLGEYFNGDLGWVEDLEAEALAVRLK
RNGRRVVIRPFVWEKIVYTYDSEREEIKPQVVGTFRQVPVRLAWALTVHKAQGLTLDKVHLELGRGLFAHGQPYVALTRV
RRLQDLSLSRPIAPTELLWRPEVEVFETRIQEGIWQKSHGWPSL