Crystal Structure of Two-Domain Laccase from Streptomyces griseoflavus produced at 0.25 mM copper sulfate in growth medium

Investigations of Accessibility of T2/T3 Copper Center of Two-Domain Laccase fromStreptomyces griseoflavusAc-993.

MDRRGFNRRVLLGGVAATTSLSIAPEAVSAPESAGTAAAAGAAPAGGEVRRVTMYAERLAGGQMGYGLEKGKASIPGPLI
ELNEGDTLHVEFENTMDVPVSLHVHGLDYEISSDGTKQNKSHVEPGGTRTYTWRTHEPGRRADGTWRAGSAGYWHYHDHV
VGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFNDMTINNRPAHTGPNFEATVGDRVEIVMITHGEYYHTFHMHGHR
WADNRTGMLTGPDDPSQVIDNKICGPADSFGFQIIAGEGVGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYDPQEH
AH

AGAAPAGGEVRRVTMYAERLAGGQMGYGLEKGKASIPGPLIELNEGDTLHVEFENTMDVPVSLHVHGLDYEISSDGTKQN
KSHVEPGGTRTYTWRTHEPGRRADGTWRAGSAGYWHYHDHVVGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFNDM
TINNRPAHTGPNFEATVGDRVEIVMITHGEYYHTFHMHGHRWADNRTGMLTGPDDPSQVIDNKICGPADSFGFQIIAGEG
VGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYDPQEHAH

0.3M NACL, 0.01 M TRIS-HCL 27,5 % (W/V) PEG 4K, PH 8

CRYSTAL STRUCTURE OF TWO-DOMAIN LACCASE FROM STREPTOMYCES GRISEOFLAVU

Two-Domain Laccase, laccase, Streptomyces griseoflavus, oxidoreductase