Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria

Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.

MGSSHHHHHHSSGLVPRGSHMLKLLEPFDLNGLELANRMVMAPLTRNRAGPRFVPTKMNALYYAQRSGLGLIITEATQIS
QQGMGYPDTPGIYTDEQVDGWRMVTEAVHRREGCIFLQLWHVGRVSHSSFQPNGQLPVAPSAIAPEGEVMTYDGIKPFET
PRALETEEVAHIVEDYRKAAINAKRAGFDGIEIHSANGYLLHEFLEDGTNKRTDRYGGSIENRARIVFEVLDAVCKVYPS
RRVGIRLSPDTEFMSMSDSDRPALYSYLVQELSRRELAYLHLIEPRIKGNVDVEKESSLNVEFFRPLYKGVLITAGGYQK
ETGEERLQKQHADLVAYGRWVIANPDLPSRFEQNAPLNPYDRATFYGGNEKGYTDYPFLDPRDSQEALKEAEAAERKWRR
L

0.2 M MgCl2 hexahydrate or 0.2 M CaCl2 dihydrate, 0.1 M MES pH 6.0, 20 % w/v PEG 6000

ene-reductase, Old Yellow Enzyme, Biocatalysis, FLAVOPROTEIN

MLKLLEPFDLNGLELANRMVMAPLTRNRAGPRFVPTKMNALYYAQRSGLGLIITEATQISQQGMGYPDTPGIYTDEQVDG
WRMVTEAVHRREGCIFLQLWHVGRVSHSSFQPNGQLPVAPSAIAPEGEVMTYDGIKPFETPRALETEEVAHIVEDYRKAA
INAKRAGFDGIEIHSANGYLLHEFLEDGTNKRTDRYGGSIENRTRIVFEVLDAVCKVYPSRRVGIRLSPDTEFMSMSDSD
RPALYSYLVQELSRRELAYLHLIEPRIKGNVDVEKESSLNVEFFRPLYKGVLITAGGYQKETGEERLQKQHADLVAYGRW
VIANPDLPSRFEQNAPLNPYDRATFYGGNEKGYTDYPFLDPRDSQEALKEAEAAERKWRRL