Crystal structure of an inverting family GH156 exosialidase from uncultured bacterium pG7

Inverting family GH156 sialidases define an unusual catalytic motif for glycosidase action.

exosialidase from uncultured bacterium pG7

MRPETIPGISLNEDNSHYFYTRAGRRLSAEEVDSWVDQYAGTQVKELMLCPNCMRTSYASQVWDPIWRGYDPAGPDDQPL
LASLPPEERVAARGWIHTAWQLHQDGIDIYARWIRRCRQRGISPWISMRMNDVHYVNDERCFLHSEFWRENPQLRRVPYR
FAEWTDRAFDYGRAEVREHHLKLIRELAARYDFDGLELDWMRFGFHFRPGYEAEGAEILTAFTAEVRRLLDDWEKRRGHK
IHLGARIPSRPATALGLGMDAVTWARRGLVDMLVITPFWASAETDMPVEIWRQLLEGTGVTLAAGLEVLLRPYPDSPLFQ
TNSLETVRGAAASLLDRGAQRIYLFNYMDSQTAMEDLENYPTLLREIGSLETLAGKPRRHVLTFADTWAPGEPRAIPLPA
TCRPGEWRAFRLHTGPKPEPGEVIAALGIEGGVAIGPETLEVRVNGELCAFLGLVDLSKPRPDFPVYGFSVPLAAMRRGY
NLIEVTARQELRFGWAEFLIRPWHSHHHHHH

0.8 M sodium formate, 15% PEG 4000, 0.1 M sodium acetate

SEC-MALLS confirmed the dimeric assembly in solution

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

(beta/alpha )8 barrel, hydrolase, sialidase, inverting, homodimer