Crystal structure of the glutamate transporter homologue GltTk in complex with D-aspartate

Binding and transport of D-aspartate by the glutamate transporter homolog Glt Tk .

MGKSLLRRYLDYPVLWKILWGLVLGAVFGLIAGHFGYAGAVKTYIKPFGDLFVRLLKMLVMPIVLASLVVGAASISPARL
GRVGVKIVVYYLATSAMAVFFGLIVGRLFNVGANVNLGSGTGKAIEAQPPSLVQTLLNIVPTNPFASLAKGEVLPVIFFA
IILGIAITYLMNRNEERVRKSAETLLRVFDGLAEAMYLIVGGVMQYAPIGVFALIAYVMAEQGVRVVGPLAKVVGAVYTG
LFLQIVITYFILLKVFGIDPIKFIRKAKDAMITAFVTRSSSGTLPVTMRVAEEEMGVDKGIFSFTLPLGATINMDGTALY
QGVTVLFVANAIGHPLTLGQQLVVVLTAVLASIGTAGVPGAGAIMLAMVLQSVGLDLTPGSPVALAYAMILGIDAILDMG
RTMVNVTGDLAGTVIVAKTEKELDESKWISHHHHHHHH

Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

20% glycerol, 10% PEG 4000, 100 mM Tris/bicine, pH 8.0, 60 mM CaCl2, 60 mM MgCl2, 0.75% n-octyl-b-D-glucopyranoside

Netherlands Organisation for Scientific Research

AMINO ACID TRANSPORTER, ASPARTATE TRANSPORT, GLUTAMATE TRANSPORTER HOMOLOGUE, TRANSPORT PROTEIN, MEMBRANE PROTEIN

MGKSLLRRYLDYPVLWKILWGLVLGAVFGLIAGHFGYAGAVKTYIKPFGDLFVRLLKMLVMPIVLASLVVGAASISPARL
GRVGVKIVVYYLATSAMAVFFGLIVGRLFNVGANVNLGSGTGKAIEAQPPSLVQTLLNIVPTNPFASLAKGEVLPVIFFA
IILGIAITYLMNRNEERVRKSAETLLRVFDGLAEAMYLIVGGVMQYAPIGVFALIAYVMAEQGVRVVGPLAKVVGAVYTG
LFLQIVITYFILLKVFGIDPIKFIRKAKDAMITAFVTRSSSGTLPVTMRVAEEEMGVDKGIFSFTLPLGATINMDGTALY
QGVTVLFVANAIGHPLTLGQQLVVVLTAVLASIGTAGVPGAGAIMLAMVLQSVGLDLTPGSPVALAYAMILGIDAILDMG
RTMVNVTGDLAGTVIVAKTEKELDESKWIS