Mechanism of eIF6 release from the nascent 60S ribosomal subunit

HIS A 259 HAS WRONG CHIRALITY AT ATOM CA

DICTYOSTELIUM 60S CARRYING ENDOGENOUS EIF6 WITH RECOMBINANT HUMAN SBDS

Eukaryotic translation initiation factor 6

Ubiquitin A-52 residue ribosomal protein fusion product 1

Shwachman-Bodian-Diamond syndrome protein

UCCGCCUCACCUUUGUAAGAUUACCCGCUGAACUUAAGCAUAUCAGUAAGCGGAGGAAAAGAAACUAACUAGGAUUCCGU
CAGUAACGGCGAGUGAAGACGGAAUAGCCCAAGGUUCAAACCUGGAUCUCUUCGAGGUUAGGUGAUGUGACCUAUGGACU
GAUGGAGCCCGCUGUUGUGACUGCUAAUUCCGUUUGGAAUUUCGAGUCGUAGAAGGUGAUAACCCUGUUCGCAGUAUCAC
AACAGUUGGACUUUGCCAUUAGCUCCACGAGUAGGAAUGUCUGAAAUUGCAUUCUGAAUGGGUGAUAAGAUUCAUCCAAG
GCUAAAUAUAUGUUAGGAGAUCGAUAGCAUACAAGUACCGUGAGGGAAAGGUGAAAAGAACUUUGAAAAAAGGUUUAAAA
GUAUUUGACACCGUUUAUGUGGAAGCGUUUACUUGGACCCCGAUUAAUGACGUCGGUUUAGCUCUAAUUCUUAGGUGGCC
AAAGUAGAGUGUUACGUGCUGAUCAAAAGGUAACGGACAUUUGAUUCAUUGGUUAUCGACGAGGAAGGUACUCUAAAUCG
GCCAGUUACUAACGGGUGAGAUCUGAUGUUUAUAAAAUGGGGGAUGAGGCUUAUCGGCUUGCUGGUGGCUCGCUCUCAAU
AAUGGAUAUUGGGUUUCAUCAAGAGUGCAAAAUGGUGGCAAUUCACUAUUAGUGGUUAUUAAUUUUGUUUGCGUGGCUUG
GCCUUGUCUACAGGUUAUCUUCGGAUGGCUUGUAGCUUUGUUGAACGCGUGGGCUUAAUGUUGUGAUUCUAGUAGCGUUA
CCAUAUCGUUAGAGUGGGUUCAAUAAAUGUCCCGUCUUGAAACACGGAUCAAGGAGGCCGUUUUGUGUGCGAGUGUAAGA
GUAAUUAAAACUCUGACGCGUAUUGAAAGAAAGAAUACUCCAAAAGAUCGUAACUACGGUUACCUUCUGUAAGGAGUGCC
CGAAUCAUGAGAACUCUGUUUCGAAAGGAUUUGCGGUUGAGCACCUAGAAUGGGACCCGAAAGGUUGUGAACUAUGCCUG
AGGAAGGCGAAGUCAGGGGAAACUCUGAUGGAGGCUUGUCGCAAUGCUGACGUGCAAAUCGCUUGUCUAACUUGGGUAUA
GGGGCGAAAGACUAAUCGAACAACCUAGUAGCUGGUUCCUUCCGAAGUUUCCCUCAGGAUAGCUGGAGCAGUAUUCUAGU
UCCAUCUUGUAAAGACAAUGAUUAGCAGUUUCGGGGGCGUAAUGCUCUCAGCUGAUUCUCAAACUCUGAACGGGUGGGUA
UCAUUUUAAUUCACUUAAUUGGAUUUUAAAAUUAAAUUGCACAUGUGCAAUGAAAAAUAGGAGCUCUUAGUGGGCCAUUU
UUGGUAAGCAGAACUGGCGAUGUGGGUUGAACCAAAUAUUGGGAUAAGACGUCUAACAUUCACUAAUAGAUACCACAAAA
GGUGUUAGUUCAUUAAGACAGCAGGACGGUGGCCAUGGAAGUCGGUAUCCGCUAAGGAGUGUGUAACAACUCACCUGCCA
AAUGGACUAGCCCUGAAAAUGGAUGACGCUAGCAGUGGAUGGUCGAUGCCCAAUCGUUAAAAGAAGUGAUAAUACUUUUA
ACGUGUAGGAAGGCGUGAAGGUAACGUAGAAGCUUGAAUGUGAAUUCGAGUGGAGUUGUCUUUAGUGCAGAUCUUGAUGG
UAGUAGCAAAUAUUCAAAAGAAUUUACUUUGAAGGCCGAAGUGGGGAAGGGUUCCAUAACAAUGGAAUUCACUUAUGGGU
GAGUCGAUCCUAAGGUUUGGGUUAACUCUCUCUAAUAAGGUUACUAGGUCAUUGGAUCGAAAGUGAAGGUGGCUUUAACA
CUAGUGACUUUAUAGGCCGAAAGGGAAGCGGGUUAAAAUUCCUGCACCAUCGAAUGGGAUAUUAGGGUAACCGAUCGUAA
UCCGGGACAUCAAUUGGCGGUCGAGGAAGAGUUAUCUUUUCUUGUUAACAUUGUCUUGGGGUCCUCCGAAUCAGGUCAAC
UGGAGACGAGGAUUCAUCGCACAAUGGAAGAGCACAGUCCUUUGGAUUGGGUCUCGCAUCCGCUAAAUGGUCCUUGAAAA
CCGGAUUAUGGUAUUUAAUCCUAUUUGGUGUUCGUACCAAUAACCACAUCAGGUCUCCAAGGUGAAUAGCCUCUGGUCAA
AUGUAUUAAUGUAGAUAAGGGAAGUCGGCAAAACCGAUCUGUAACUUCGGGAUAAGGAUUGGCUCUAAAGGCUGGUGGAG
UGGACAUAUUGGAGUUUGCUAUUUGUUUUUUACUUUUAGGAUGGGCAACUGUUUUGAAGGUUUAAGAUGGGUGGUAAUUC
UUUCCAAUGUGAGGGCUUGCUCGUUCUGCUUUACGAUUAACAGCUAAUUUAGAACUGUGACGAUCACCGGGAAUCCAACU
GUUUAAUUAAAACAAAGCAUUGCGAUAAGCUUAAAAGCUUUUGACGCAAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAA
AGUGAAGAGAUUCAACCUAGCACGGGUAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCU
AAUUAGUGACGCGCAUGAAUGGAUCAAUGAGAUUCCCACUGUCCCUAACUACUAUACAGCGAAACCACUGCAAGGGGAAC
GGGCCUUGCAAAAACAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUCUGAUAUUGCAUAGUGACCUAAAAGGUG
UAGAAUAGGUGGGAGGGGCAACCCGACGGUGAAAUACCACCCCUUUUGGCGUUACUUUGCUAACUUGGAAUAACAGUACC
UCAUAAUUCAUUUUAUGAUGGUUUUGGUGAAUAAGCGGAUCAACCACGGGUGAAAUCUGUGCAAAUUGGGCAACUGAUUU
GUAUAGCAAAGUAGUCCCUCUGGUCCCGUAUUAUGUCGACCAAGAACAGUUUCAGGUGGGGAGUUUGGCUGGGGCGGCAC
AUUUGUUAAAAGAUAACGCAAGUGUCCAAAGGCAGGCUCAGUGAGAACAGAAAUCUCACGUAGAGUAAAAGGGCAAAAGC
CUGCUUGAUUCUGAUUUUCAGUACUAAUCGGAACUGGGAAACCAGGGCCUAUCGAUCCUUUAUGUGCUUAAAUCUUAACC
CUAGAGGUGUCAGAAAAGUUACCACAGGGAUAACUGGCUUGUGGCAGCCAAGCGCUCAUAGCGACGCUGCUUUUUGAUCC
UUCGAUGUCGGCUCUUCUUAUCAUUGUGAAGCAGAAUUCACAAAGUGUUGGAUUGUUCACCCACUAACAAGGAACGUGAG
CUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUACCCUACUGUUGUCAAUUGUUUGCGUAAUAGUAGCAUGAUUUAGUA
CGAGAGGAACUGUCAUGCCGGAUCACUGGUCUGUAGGUUUAUUUGACAAAAUAGUGACCUGCCGCUACCAUCCGUUGGAU
AAUGGCUGAACGCCUCUAAGUCAGAAUCCAUUCUAGAAACGCAAACCAAAUGCUUUAGAGUGUGAAUGUUGUAGGUAACA
UUAGGUUGUUGGUGGGGGACCACUUUCAACUUUAAACCAUAUGAUUAAUCGCUGUUACACUGCAGUUUCCUUCCGGUUAU
UGUGGUGGGUGGCUAAAUUCUAAUUUAUAUCCUCGUUCCGCUCAACUCUUCGAUUGUAGACGACUAUCAAAUGAACUAGG
UGCUGUAAGCUUCCGAGUAGCGUUCAGUUACGAGGGGUUGAGGCUUUUCCAUUAGUUCUUU

MSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGMTHVVRDLEKPGSKMHKKEIVEAVTIIE
CPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDNFRRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSV
VRVIAHTQVHKLKLTQKKAHVLEIQVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDVIGVTKGKGFNGVIKRWGVRK
LPRKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVERNKKIYRIGQAQPEDGKQISTGKTEFDLTEKTINPMGGFA
HYGMVKHEFLMLKGCVAGPRKRALTLRKSITTQTGRAALEKITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA

MKVINSSRKVQIPENVTVDVKGRSVKVTGPRGTLSKSFDHASVDINLVGKKELTVDLWFGNRKQIACIKTITSIIENMIT
GVTKGYEYKMRFVYAHFPINVAVTDGGRVVEIRNFFGEKIVRRIELLDGITCYRNEKAKDEIVLTGNSLELLSQSCATIQ
LRSAIKYKDVRKFLDGIYVSERNVLESN

MSKAQAVGSNYRVSLGLPVGAVMNSADNSGAKNLYVIAVKGIKGRLNRLPSAGVGDMVMATVKKGKPELRKKVCTGLVVR
QRKHWKRKDGVYIYFEDNAGVMCNPKGEVKGNILGPVAKECSDLWPKVATNAGTIV

MGRRPARCYRYCKNKPYIKSRYCRGVPDAKIRIFDLGRKKASTDEFPLCVHLISLEKEQLSSEAIEAGRISCNKYISKTG
GKDSFHMRVRVHPWHVLRINKMLSCAGADRLQTGMRGAFGKPMGTVARVNIGQIIFSIRTRDNMLANVVEALRRSSYKFP
GRQKIVVSKKWGFTAYNREAYQKLKADGRLMNDGANVKVITNHGTLAQYAKDIAAAN

MATRLQYENSCDVGVFLKLTNKYCLVGQCGSKQFLHTVENRLADHIPVVETSIAGTRIVGRLSAGNKNGLLLPNTCTDQE
LQQIRNSLPDDVVVQRIEEKFSALGNCIATNDYVALVHPDIDRETEEIIADVLGVEVFRQTVSGNVLVGTYCALTNQGAL
VHPMTSIADQDELSSLLQVPLVAGTVNRGNECVAAGCVVNDWTAIVGADTTATEISVIESIFAL

MSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQ
TEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQ
LKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIESEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLK
DVEEGDEKFE

MSGAGSKRKNVFIEKATKLFTTYDKMIVAEADFVGSSQLQKIRKSIRGIGAVLMGKKTMIRKVIRDLADSKPELDALNTY
LKQNTCIIFCKDNIAEVKRVINTQRVGAPAKAGVFAPNDVIIPAGPTGMEPTQTSFLQDLKIATKINRGQIDIVNEVHII
KTGQKVGASEATLLQKLNIKPFTYGLEPKIIYDAGACYSPSISEE

MPPKVDPSEKVEVFLRVCGGEAGAMSTLAPKLGPLGVSPKKVGDDIAKATQPWKGMKVSVKLTIQNRIAVPEVLPSASAL
VIKALKEPPRDRKKEKNIKHNGNIPLEEICKIAKTMRFKSLAVDFKGSVLEILGTAHSVGCKVNGKSPRDIQAGIQSGEI
EVVEPK

Medical Research Council (United Kingdom)
60S ribosomal protein L3, 60S ribosomal protein L9, 60S ribosomal protein L23, 60S ribosomal protein L10, 60S ribosomal protein L24, Ubiquitin-60S ribosomal protein L40, Eukaryotic translation initiation factor 6, Ribosome maturation protein SBDS, 60S acidic ribosomal protein P0, 60S ribosomal protein L12/RNA Complex