The structure of Schizosaccharomyces pombe PCNA in complex with an Spd1 derived peptide

MLEARFQQAALLKKLLDAIKELVTDANFDCNDNGISLQAMDSSHVALVSMLIKSDGFEPYRCDRNIALGINLNALSKVLR
CAQNEDLVTLKAEDTPEVLNLVFESEKNDRISDYDVKLMDIDQEHLGIPDIEYDATITMPAAEFQRITRDLLTLSDSVTI
NASKEGVRFSCKGDIGNGSTTLKQHTDLSDQDQSIEISLTQAVTLTFSLKYLAQFTKATPLATRVTLSMSNDVPLLVEYK
MESGFLRFYLAPKIGEEDEE

100 mM TRIS-HCl pH 7.5, 18% (w/v) PEG4000 and 6% (v/v) glycerol, in the presence of 1.9 mM Spd1(27-46).

[PCNA]=0.17 mM (5 mg/mL)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

DNA repair, DNA replication, cell cycle regulation, PCNA-Spd1 complex, DNA BINDING PROTEIN