MloK1 model from single particle analysis of 2D crystals, class 2 (intermediate conformation)

Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.

The consensus model was flexibily fit in to the EM map of this class using Normal Mode Analysis with iMODFIT, and then real-space refined using PHENIX.

20 mM KCl, 20 mM Tris-HCl pH 7.6, 1 mM BaCl2, 1 mM EDTA, 0.2 mM cAMP

The defocus at the center of each particle box was estimated based on the tilt geometry.

MloK1 potassium channel in lipidic bilayer of 2D crystals

The dataset of 346 movies recorded and processed for Kowal et al. (2018) 2 was employed. As reported, the data were collected on an FEI Titan Krios TEM equipped with a Gatan K2 DED. Total dose: 40 e-/A^2 distributed over 40 movie frames. Pixel size: 1.3 A on the sample level (counting mode). Nominal tilt range: -55 to +55 degrees.

The particle positions were given by the cross-correlation profile generated by the classical unbending algorithm.

Cyclic nucleotide-gated potassium channel mll3241

MSVLPFLRIYAPLNAVLAAPGLLAVAALTIPDMSGRSRLALAALLAVIWGAYLLQLAATLLKRRAGVVRDRTPKIAIDVL
AVLVPLAAFLLDGSPDWSLYCAVWLLKPLRDSTFFPVLGRVLANEARNLIGVTTLFGVVLFAVALAAYVIERDIQPEKFG
SIPQAMWWAVVTLSTTGYGDTIPQSFAGRVLAGAVMMSGIGIFGLWAGILATGFYQEVRRGDFVRNWQLVAAVPLFQKLG
PAVLVEIVRALRARTVPAGAVICRIGEPGDRMFFVVEGSVSVATPNPVELGPGAFFGEMALISGEPRSATVSAATTVSLL
SLHSADFQMLCSSSPEIAEIFRKTALERRGAAASA

Voltage-gated potassium channel, Cyclic nucleotide-binding domain, ion channel, ion transport, MEMBRANE PROTEIN