Crystal structure of the soluble domain (residues 71-217) of a conserved hypothetical secreted protein (Rv2700 ortholog) from Mycobacterium marinum
Seattle Structural Genomics Center for Infectious Disease (SSGCID)
SSGCID, Mycobacerium marinum, Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, secreted protein, Rv2700 ortholog
MAHHHHHHKLGERVSRTEMTDVTPAQLGETKVRVLNASGRGGQAADVAGALKDLGFTQPTAANDPVYADTRLDCQGQIRF GTAGQATAAAVWLVAPCTELFNDGRADDSVDLVLGTDFTTLAHNDDIDAVLSSLRPGATQPPDPTLIAKIHASSC
Mycobacterium marinum (strain ATCC BAA-535 / M)
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Hampton Research Index screen, condition H1 (305884h1): 100mM Citric acid pH 3.5, 2000mM Ammonium sulfate: MymaA.19257.a.B3.PS38530 at 22.5mg/ml cryo: 25% EG: tray 305841 H1: puck bqx9-4. For experimental phasing, a crystal from the same condition, Index H1 (305884h1), was soaked for 15 seconds each in a mix of 87.5% reservoir / 12.5% 2.5M sodium iodide in ethylene glycol, followed by a soak in 75% reservoir / 25% 2.5M sodium iodide in ethylene glycol, vitrified in liquid nitrogen for in-house data collection at Cu-Kalpha wavelength: puck vgz4-3
Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases
SSGCID, Mycobacerium marinum, Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, secreted protein, Rv2700 ortholog, UNKNOWN FUNCTION
KLGERVSRTEMTDVTPAQLGETKVRVLNASGRGGQAADVAGALKDLGFTQPTAANDPVYADTRLDCQGQIRFGTAGQATA AAVWLVAPCTELFNDGRADDSVDLVLGTDFTTLAHNDDIDAVLSSLRPGATQPPDPTLIAKIHASSC