Remarkable rigidity of the single alpha-helical domain of myosin-VI revealed by NMR spectroscopy
Remarkable Rigidity of the Single alpha-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy.
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
15N-, 13C-, and 2H-isotopically enriched
1-mM sample of perdeuterated, 15N-enriched MT sample in 95% H2O, 5% D2O, 25 mM sodium phosphate buffer, pH 7.8
13 mg/mL Pf1 in 25 mM sodium phosphate buffer, pH 6.3, 2 mM EDTA
13 mg/mL Pf1 in 25 mM sodium phosphate buffer, pH 6.3, 2 mM EDTA, an additional 100 mM NaCl
1 mM U-15N; U-13-; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O
1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
myosin-VI, alpha-helical domain, MOTOR PROTEIN