The Structure of EgtB(Cabther) in complex with Hercynine

Crystal Structure of the Ergothioneine Sulfoxide Synthase fromCandidatus Chloracidobacterium thermophilumand Structure-Guided Engineering To Modulate Its Substrate Selectivity.

MGDRGPEFEAARSHPEPIQSGEVSDRKAWQRHYRAVRAVSEAICQPLETEDYVVQPMPDVSPPKWHLGHTSWFFETFILK
SGLADYRPFHPRYDYIFNSYYEAVGARHPRPQRGLLTRPTVSEVYAYRAHVDAAVERFIAHSDTRTWAALQPILELGLHH
EQQHQELLLTDIKAILATNPLDPVYRPQPQPLPSPVEQLSPTGDWHIVEGGRYAIGHAGRGFAFDNEGPRHDVLLRPCRI
AARPVTNGEFLAFMADGGYRRPELWLSDGWAAVTARGWEAPLYWRQAADGTWETLTLHGVQPVAPYEPVCHISFYEADAY
ARWAGKRLPTEAEWEVVAARLPVTGNFYESGVLHPRPVSVSAAFYGDVWVWTASPYVGYPGFRPVSGALGEYNGKFMCNQ
MVLRGGSCATSLTHIRSTYRNFFPPDARWQFTGVRLAEDMSLEVDLQGDHGLSAWSHPQFEK

Chloracidobacterium thermophilum (strain B)

0.2M Na Acetate, 0.1M Tris Acetate pH 6.5 and 5% PEG 4000

non-heme iron dependent enzyme, Ergothioneine, METAL BINDING PROTEIN

EAARSHPEPIQSGEVSDRKAWQRHYRAVRAVSEAICQPLETEDYVVQPMPDVSPPKWHLGHTSWFFETFILKSGLADYRP
FHPRYDYIFNSYYEAVGARHPRPQRGLLTRPTVSEVYAYRAHVDAAVERFIAHSDTRTWAALQPILELGLHHEQQHQELL
LTDIKAILATNPLDPVYRPQPQPLPSPVEQLSPTGDWHIVEGGRYAIGHAGRGFAFDNEGPRHDVLLRPCRIAARPVTNG
EFLAFMADGGYRRPELWLSDGWAAVTARGWEAPLYWRQAADGTWETLTLHGVQPVAPYEPVCHISFYEADAYARWAGKRL
PTEAEWEVVAARLPVTGNFYESGVLHPRPVSVSAAFYGDVWVWTASPYVGYPGFRPVSGALGEYNGKFMCNQMVLRGGSC
ATSLTHIRSTYRNFFPPDARWQFTGVRLAEDMS