A sub-atomic resolution cryo-EM of full-length Rotavirus A-VP3 (RVA-VP3)

EMAN2 e2boxer.py was uesed to automatically select particle images

MKVLALRHSVAQVYADTQIYTHDDTKDSYENAFLISNLTTHNILYFNYSARTLEILNKSGIAAIEIQSLEELFTLIRCNF
TYDYENNVVYLHDYSYYTNNEIRTDQHWITKTNIEEYLLPGWKLTYVGYNGNDTRGHYNFSFTCQNAATDDDIIIEYIYS
EALDFQNFMLRKIKERMTTSLPIARLSNRVFRDKLFPLLVKKHKRVVNVGPRNESMFTFLNFPSIRQFSNGPYLVKNTIK
LKQERWLGKRVSQFDIGQYKNMLNVITTIYHYYNLYQEKPIIYMVGSAPSYWIYDVRQYSDFLFETWDPLDTPYSSIHHK
ELFFAKDIGKLKDNSILYIDIRTDRGNADWKEWRKVVELQTISNLNLAYQYLATGKSKVCCVKLTAMDLELPVSAKLLHH
PTTEIRSEFYLLLDIWDVNNIKRFIPKGALYSFINNVITDNVFIQSPFKIRTSVSDYIVALYALSNDFNNREDIINLINN
QKQSLITVRINNTFKDEPKVGFKSIYDWTFLPTDFETTNAIVTSYDGCLGIFGLSISLASKPTGNNHLFILNGTDKYYKL
DQFANHTGISRRSHQVRFSESATSYSGYIFRDLSNSNFNLIGTNVENSVSGHVYNALIYYRYNYSFDLKRWIYLHSVEKA
NIEGGKYYEHAPIELIYACKSAKEFASLQDDLTVLRYANEIENYINKVYSITYADDPNYFIGIKFNNIPYIYDVKVPHLT
FGVLYISDNMIPDVVKIMKSMKQELFGMDVTTSYTYMLSDGVYVANVSGVLATYFKMYNLFYKNQITFGQSRMFIPHITL
SFSNNKTVRIETTKLRIKSIYLRKIRGDTVFDMPE

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Gel filtration profile indicates a tetrameric assembly in solution
Analytical Ultracentrifugation confirms tetrameric state is preferred oligomeric assembly in solution
Rotavirus, Capping enzyme, Methyl transferase, RTPase, PDE, STRUCTURAL PROTEIN