Tar14, tryptophan C-6 flavin-dependent halogenase (chlorinase) from taromycin biosynthesis

Biosynthesis of l-4-Chlorokynurenine, an Antidepressant Prodrug and a Non-Proteinogenic Amino Acid Found in Lipopeptide Antibiotics.

GSHMSVSGSERSAEGNRKKRVVIVGGGTAGWMTASYLTAAFGDRVDLTVVESAQIGTIGVGEATFSDIRHFFEFLRLEES
DWMPECNATYKLAVRFENWREPGHHFYHPFEQMSSVDGFPLSDWWLRNPTTSRFDKDSFVMTSLCDAGVSPRYLDGSLID
QDFVEQERDDDSARSTIAEYQGAQFPYAYHFEAHLLAKYLTGYATRRGTRHIVDNVVDVALDERGWISHVRTEEHGDLEA
DLFVDCTGFRGLLLNKALGEPFVSYQDTLPNDSAVALQVPLDMEREPIRPCTTATAQEAGWIWTIPLISRVGTGYVYASD
YTTPEQAERVLRDFVGPAAADVPANHIKMRIGRSRRSWVNNCVGVGLSSGFVEPLESTGIFFIHHAIEQIVKYFPSGGAG
DDRLRELYNRSVGHVMDGVREFLVLHYRSAKRADNQYWKDTKTRTVPDSLAERIEFWKHKVPDAETVYPYYHGLPPYSYN
CILLGMGGIDVNYSPALDWANEKAALAEFERIRVKAEKLVQELPTQNEYFAAMRAGR

0.1 M BisTris methane (pH 6.5), 0.35 M Li2SO4, 28% PEG 3350

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

halogenase, flavin, tryptophan, taromycin, BIOSYNTHETIC PROTEIN

MSVSGSERSAEGNRKKRVVIVGGGTAGWMTASYLTAAFGDRVDLTVVESAQIGTIGVGEATFSDIRHFFEFLRLEESDWM
PECNATYKLAVRFENWREPGHHFYHPFEQMSSVDGFPLSDWWLRNPTTSRFDKDSFVMTSLCDAGVSPRYLDGSLIDQDF
VEQERDDDSARSTIAEYQGAQFPYAYHFEAHLLAKYLTGYATRRGTRHIVDNVVDVALDERGWISHVRTEEHGDLEADLF
VDCTGFRGLLLNKALGEPFVSYQDTLPNDSAVALQVPLDMEREPIRPCTTATAQEAGWIWTIPLISRVGTGYVYASDYTT
PEQAERVLRDFVGPAAADVPANHIKMRIGRSRRSWVNNCVGVGLSSGFVEPLESTGIFFIHHAIEQIVKYFPSGGAGDDR
LRELYNRSVGHVMDGVREFLVLHYRSAKRADNQYWKDTKTRTVPDSLAERIEFWKHKVPDAETVYPYYHGLPPYSYNCIL
LGMGGIDVNYSPALDWANEKAALAEFERIRVKAEKLVQELPTQNEYFAAMRAGR