X-ray structure of PEDV papain-like protease 2

Center for Structural Genomics of Infectious Diseases (CSGID)

Evaluating ubiquitin specificity of papain-like protease 2 from alphacoronaviruses FIPV and PEDV using structure-guided engineering

SNNWDSHYGFDKAGEFHMLDHTGFAFPSEVVNGRRVLKTTDNN(CSO)WVNVTCLQLQFARFRFKSAGLQAMWESYCTGD
VAMFVHWLYWLTGVDKGQPSDSENALNMLSKYIVPAGSVTIERVTHDGCCCSKRVVTAPVVNASVLKLGVEDGLCPHGLN
YIDKVVVVKGTTIVVNVGKPVVAPSHLFLKGVSYTTFLDNGNGVAGHYTVFDHDTGMVHDGDVFVPGDLNVSPVTNVVVS
EQTAVVIKDPVK

SNNWDSHYGFDKAGEFHMLDHTGFAFPSEVVNGRRVLKTTDNNCWVNVTCLQLQFARFRFKSAGLQAMWESYCTGDVAMF
VHWLYWLTGVDKGQPSDSENALNMLSKYIVPAGSVTIERVTHDGCCCSKRVVTAPVVNASVLKLGVEDGLCPHGLNYIDK
VVVVKGTTIVVNVGKPVVAPSHLFLKGVSYTTFLDNGNGVAGHYTVFDHDTGMVHDGDVFVPGDLNVSPVTNVVVSEQTA
VVIKDPVK

0.1 M HEPES, pH 7.5, 1.3 M K/Na tartrate

Center for Structural Genomics of Infectious Diseases
NIAID, National Institute of Allergy and Infectious Diseases

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Oligomers of PEDV PLP2 form at high concentrations by SDS-PAGE analysis. PEDV PLP2 elutes in one single peak using size exclusion chromatography.

papain-like, viral protein, protease, HYDROLASE, Structural Genomics, Center for Structural Genomics of Infectious Diseases, CSGID

NWDSHYGFDKAGEFHMLDHTGFAFPSEVVNGRRVLKTTDNNCWVNVTCLQLQFARFRFKSAGLQAMWESYCTGDVAMFVH
WLYWLTGVDKGQPSDSENALNMLSKYIVPAGSVTIERVTHDGCCCSKRVVTAPVVNASVLKLGVEDGLCPHGLNYIDKVV
VVKGTTIVVNVGKPVVAPSHLFLKGVSYTTFLDNGNGVAGHYTVFDHDTGMVHDGDVFVPGDLNVSPVTNVVVSEQTAVV
IKDPVK