Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ1 in complex with ZIKV E glycoprotein

Potent Zika and dengue cross-neutralizing antibodies induced by Zika vaccination in a dengue-experienced donor.

QVQLQESGPGLVKSSETLSLSCTVSGASISNFYWSWVRQPPGKGLEWMGSIYYTGTITYNPSLKSRLTMSVDTSKNQFSL
KLNSVTAADTAVYSCAGLDRFNWNDEGDCWGQGTLVTVFSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVS
WNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK

QSVLTQPPSASGTPGQRVTISCSGSRSNLGRNTVNWYQQLPGVAPKLLIYSNNRRPSGVPDRFSGSKSDTSASLAISGLQ
SEDEADYFCAAWDDSLNGLYVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPV
KAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC

IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEA
YLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET
DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKE
ALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAE
TLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHW
HRSGSGPLEVLFQGPGSAWSHPQFEKGGGSGGGSGGGSAWSHPQFEK

isolate ZIKV/Human/French Polynesia/10087PF/2013
Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013)

0.06 M MgCl2, 0.1M Imidazole
MES monohydrate (pH 6.5), 20% Ethylene glycol and 10% PEG 8,000

Assembly was tested by gel-filtration and native PAGE

ZIKV-DENV, Antibody, human, Vaccination, VIRAL PROTEIN-IMMUNE SYSTEM complex

(chain A and (resid 1 through 139 or resid 141 through 195 or resid 197 through 214))

(chain H and (resid 1 through 139 or resid 141 through 195 or resid 197 through 214))

IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEA
YLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET
DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKE
ALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAE
TLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHW
HRSGS