Structure of the transmembrane domain of the Death Receptor 5 mutant (G217Y) - Trimer Only
Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signaling.
Tumor necrosis factor receptor superfamily member 10B
Death receptor 5,TNF-related apoptosis-inducing ligand receptor 2,TRAIL-R2
TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Transmembrane Domain of DR5 Mutant G217Y
0.8 mM [U-13C; U-15N; 85%-2H] Transmembrane Domain of DR5 Mutant G217Y, 50 mM DMPC, 100 mM DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O
(15N, 13C, 85% 2H) labeled sample for backbone resonance assignment
0.8 mM [U-13C; U-15N] Transmembrane Domain of DR5 Mutant G217Y, 50 mM [acyl chain U-2H] DMPC, 100 mM [acyl chain U-2H] DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O
(15N, 13C) labeled sample for NOE assignments
0.4 mM [15%-13C; U-15N; H-2H] Transmembrane Domain of DR5 Mutant G217Y, 50 mM [acyl chain U-2H] DMPC, 100 mM [acyl chain U-2H] DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O
Isotope mixed sample for detecting inter-chain NOEs
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
OG-label method (Chen et al: The Unusual Transmembrane Partition of the Hexameric Channel of the Hepatitis C Virus. Structure, 26(4):627-634 (2018).)
transmembrane helix trimer, IMMUNE SYSTEM