Designed repeat protein specifically in complex with Fz7CRD

Receptor subtype discrimination using extensive shape complementary designed interfaces.

MGSELGTRLIRAALDGNKDRVKDLIENGADVNASLMSGATPLHAAAMNGHKEVVKLLISKGADVNAQSVAGSTPLDAAAF
SGHKEVVKLLISKGADVNAVNAAGLTPLHDAADDGHNEVVKLLISKGADVNAKADHGMTPLHFAAQRGHKEVVKLLISKG
ADLNTSAKDGATPLDMARESGNEEVVKLLEKQLEHHHHHH

HGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCE
RARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQHHHHHH

0.2 M Na2HPO4, citric acid, pH 4.2 and 2M Ammonium sulfate

National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)

Frizzled, Designed protein, BIOSYNTHETIC PROTEIN, BIOSYNTHETIC PROTEIN-SIGNALING PROTEIN complex

HGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCE
RARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQ