Structure of a peptide-based photo-affinity cross-linker with Herceptin Fc

Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.

THTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRV
VSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE
SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG

100mM sodium acetate pH=5.6, 12%(w/v) PEG 1000

( CHAIN A AND RESID 341:441 ) OR ( CHAIN E AND RESID 1:13 )

( CHAIN B AND RESID 341:441 ) OR ( CHAIN F AND RESID 1:13 )

( CHAIN A AND RESID 601:677 ) OR ( CHAIN B AND RESID 601:689 ) OR ( CHAIN E AND RESID 101:101 ) OR ( CHAIN F AND RESID 101:103 )

Immunoglobulin G1 FC, Photo-affinity peptide

Crosslinking, antibody, photo-reactive, IMMUNE SYSTEM