Cryo-EM structure of the HO BMC shell: subregion classified for BMC-T: TD-TSTSTS

The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.

The selected particle subset was refined without masking and subsequently masked to reveal only the subregion of the BMC shell to which the focused classification had been applied.

Initial CTF fitting using CTFFIND4, CTF correction applied within RELION

Bacterial microcompartment shell from Haliangium ochraceum

Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)

928 images retained after inspection for image quality.

GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER

1000 particles were picked manually to generate reference templates for subsequent auto-picking in RELION 1.4.

5-7 seconds incubation of the sample on the grid before blotting and plunging

MSITLRTYIFLDALQPQLATFIGKTARGFLPVPGQASLWVEIAPGIAINRVTDAALKATKVQPAVQVVERAYGLLEVHHF
DQGEVLAAGSTILDKLEVREEGRLKPQVMTHQIIRAVEAYQTQIINRNSQGMMILPGESLFILETQPAGYAVLAANEAEK
AANVHLVNVTPYGAFGRLYLAGSEAEIDAAAEAAEAAIRSVSGVAQESFRDR

MADALGMIEVRGFVGMVEAADAMVKAAKVELIGYEKTGGGYVTAVVRGDVAAVKAATEAGQRAAERVGEVVAVHVIPRPH
VNVDAALPLGRTPGMDKSA

MDHAPERFDATPPAGEPDRPALGVLELTSIARGITVADAALKRAPSLLLMSRPVSSGKHLLMMRGQVAEVEESMIAAREI
AGAGSGALLDELELPYAHEQLWRFLDAPVVADAWEEDTESVIIVETATVCAAIDSADAALKTAPVVLRDMRLAIGIAGKA
FFTLTGELADVEAAAEVVRERCGARLLELACIARPVDELRGRLFF

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Department of Energy (DOE, United States)

microcompartment, shell, compartmentalization, BMC fold, STRUCTURAL PROTEIN