Structure of the major pilin protein (T-18.1) from Streptococcus pyogenes serotype MGAS8232

Group AStreptococcusT Antigens Have a Highly Conserved Structure Concealed under a Heterogeneous Surface That Has Implications for Vaccine Design.

ETAGVIDGSTLVVKKTFPSYTDDKVLMPKADYTFKVEADDNAKGKTKDGLDIKPGVIDGLENTKTIHYGNSDKTTAKEKS
VNFDFANVKFPGVGVYRYTVSEVNGNKAGIAYDSQQWTVDVYVVNREDGGFEAKYIVSTEGGQSDKKPVLFKNFFDTTSL
KVTKKVTGNTGEHQRSFSFTLLLTPNECFEKGQVVNILQGGETKKVVIGEEYSFTLKDKESVTLSQLPVGIEYKVTEEDV
TKDGYKTSATLKDGDVTDGYNLGDSKTTDKSTDEIVVTNKRD

10% w/v PEG 20000, 20% v/v PEG MME 550, 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-Propanediol, 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 0.1 M BICINE/Tris base pH 8.5

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

Major pilin backbone protein, T-antigen, T18.1, STRUCTURAL PROTEIN

ETAGVIDGSTLVVKKTFPSYTDDKVLMPKADYTFKVEADDNAKGKTKDGLDIKPGVIDGLENTKTIHYGNSDKTTAKEKS
VNFDFANVKFPGVGVYRYTVSEVNGNKAGIAYDSQQWTVDVYVVNREDGGFEAKYIVSTEGGQSDKKPVLFKNFFDTTSL
KVTKKVTGNTGEHQRSFSFTLLLTPNECFEKGQVVNILQGGETKKVVIGEEYSFTLKDKESVTLSQLPVGIEYKVTEEDV
TKDGYKTSATLKDGDVTDGYNLGDSKTTDKSADEIVVTNKRD