Crystal structure of the dimeric bH1-Fab variant [HC-Y33W,HC-D98M,HC-G99M]

Binding symmetry and surface flexibility mediate antibody self-association.

anti-VEGF-A Fab fragment bH1 heavy chain

anti-VEGF-A Fab fragment bH1 light chain

EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTWIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAY
LQMNSLRAEDTAVYYCSRWGGMMFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVS
WNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTGHHHHHHHHG

DIQMTQSPSSLSASVGDRVTITCRASQDIPRSISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTIS
SLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQS
GNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

0.1 M tris-sodium citrate, pH 5.6, 11% PEG4000, 8% isopropanol

bH1-Fab variant [HC-Y33W,HC-D98F,HC-G99M]

bH1-Fab variant [HC-Y33W,HC-D98M,HC-G99M,LC-S30bR]

bH1-Fab variant [HC-Y33W,HC-D98F,HC-G99M,LC-S30bR]

DLS and ITC data also support the assembly

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

Fab fragment, antibody self-assembly, aggregation, IMMUNE SYSTEM

WGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVV
TVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKT

EIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKA
DYEKHKVYACEVTHQGLSSPVTKSFNRGEC