Crystal structure of the GH43 BlXynB protein from Bacillus licheniformis

Structure-guided design combined with evolutionary diversity led to the discovery of the xylose-releasing exo-xylanase activity in the glycoside hydrolase family 43.

MGSSHHHHHHSSGLVPRGSHMASMSGEHTYTNPVLTGFHPDPSIIRVGEDYYMVNSTFQYFPAIVISHSKDLVHWKIIGH
GITENEGLDLSDINDSHGIWAPDISYHNGTFYIFATHRLNGPTVINGRKLIRRQIMIKSSRPEGPYSKPVFIDEGSGIDP
SHFVDGDGKHYMLLSPACTLFPLNEECTDISGEPVQIWEGTGRRAPEGPHLLKKDGYYYAILAEGGTGYSHSITTARSTH
LYGPYEPCPYNPILTQTDPDAPIQRAGHGKLVETQNGEWWAVYLCGRPNQGSYTTVGRETALDPVEWTDDGWFVINNLKG
PSLVQRAPNLPQVKWDEKNFDDFDEDTLGLDWQFVRNPDHSSWSLIERPGYLRLWTGDWDLHDIRAKNTVVRREKHHLYS
AGVKLDFSPSASGEQAGIVCYYSTNNYLKCCLIYEEGLKIKVVENRSGCQKTLGKKHAEAGPLFLKAVINKQKRDFYYSY
EGKHWHHAGGTEDASFLSDEGSRDAKGHTGTMVGIFANNGGSGRKAAADFDWFRYIAY

Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)

36% (v/v) 2-methyl-2,4-pentanediol, 5% (w/v) polyethelyne glycol 8,000 and 0.1 M sodium cacodylate pH 6.0

glycoside hydrolase, GH43, Bacillus lincheniformis, HYDROLASE

MSGEHTYTNPVLTGFHPDPSIIRVGEDYYMVNSTFQYFPAIVISHSKDLVHWKIIGHGITENEGLDLSDINDSHGIWAPD
ISYHNGTFYIFATHRLNGPTVINGRKLIRRQIMIKSSRPEGPYSKPVFIDEGSGIDPSHFVDGDGKHYMLLSPACTLFPL
NEECTDISGEPVQIWEGTGRRAPEGPHLLKKDGYYYAILAEGGTGYSHSITTARSTHLYGPYEPCPYNPILTQTDPDAPI
QRAGHGKLVETQNGEWWAVYLCGRPNQGSYTTVGRETALDPVEWTDDGWFVINNLKGPSLVQRAPNLPQVKWDEKNFDDF
DEDTLGLDWQFVRNPDHSSWSLIERPGYLRLWTGDWDLHDIRAKNTVVRREKHHLYSAGVKLDFSPSASGEQAGIVCYYS
TNNYLKCCLIYEEGLKIKVVENRSGCQKTLGKKHAEAGPLFLKAVINKQKRDFYYSYEGKHWHHAGGTEDASFLSDEGSR
DAKGHTGTMVGIFANNGGSGRKAAADFDWFRYIAY