Structure of the Bovine p85a BH domain R228E mutant

Insight into the PTEN - p85 alpha interaction and lipid binding properties of the p85 alpha BH domain.

Phosphatidylinositol 3-kinase regulatory subunit alpha

PtdIns-3-kinase regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha,PtdIns-3-kinase regulatory subunit p85-alpha

GPLGSTEADSQQASTLPDLAEQFAPPDVAPPLLIKLVEAIEKKGLE(CSO)STLYRTQSSSNPAELRQLLDCDTASLDLE
MFDVHVLADAFKRYLLDLPNPVIPVAVSSELISLAPEVQSSEEYIQLLKKLIESPSIPHQYWLTLQYLLKHFFKLSQTSS
KNLLNARVLSELFSPLLFRFPAASSENTEHLIKIIEILISTEWNERQPAPALPPKPPKPTTVAN

GPLGSTEADSQQASTLPDLAEQFAPPDVAPPLLIKLVEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDTASLDLEMFDV
HVLADAFKRYLLDLPNPVIPVAVSSELISLAPEVQSSEEYIQLLKKLIESPSIPHQYWLTLQYLLKHFFKLSQTSSKNLL
NARVLSELFSPLLFRFPAASSENTEHLIKIIEILISTEWNERQPAPALPPKPPKPTTVAN

1.5 M Li2SO4, 4% glycerol, 0.1 Sodium Cacodylate buffer

Canadian Institutes of Health Research (CIHR)

Natural Sciences and Engineering Research Council (NSERC, Canada)

GAP protein, SIGNALLING PROTEIN, SIGNALING PROTEIN

QQASTLPDLAEQFAPPDVAPPLLIKLVEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDTASLDLEMFDVHVLADAFKRY
LLDLPNPVIPVAVSSELISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSELFS
PLLFRFPAASSENTEHLIKIIEILISTEWNERQPAPALPPKPPKPTTVAN