Crystal structure of the periplasmic Lysine-, Arginine-, Ornithine-binding protein (LAO) D11A mutant from Salmonella typhimurium complexed with arginine

The interplay of protein-ligand and water-mediated interactions shape affinity and selectivity in the LAO binding protein.

Lysine/arginine/ornithine-binding periplasmic protein

ALPQTVRIGTATTYAPFSSKDAKGEFIGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
IAFSDKLYAADSRLIAAKGSPIQPTLESLKGKHVGVLQGSTQEAYANDNWRTKGVDVVAYANQDLIYSDLTAGRLDAALQ
DEVAASEGFLKQPAGKEYAFAGPSVKDKKYFGDGTGVGLRKDDTELKAAFDKALTELRQDGTYDKMAKKYFDFNVYGD

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

0.2 M Sodium acetate trihydrate
0.1 M Sodium cacodylate trihydrate
30% w/v Polyethylene glycol 8,000

Periplasmic Binding Protein, LAO, Thermodynamics, Protein Ligand Complex, PROTEIN BINDING

ALPQTVRIGTDTTYAPFSSKDAKGEFIGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
IAFSDKLYAADSRLIAAKGSPIQPTLESLKGKHVGVLQGSTQEAYANDNWRTKGVDVVAYANQDLIYSDLTAGRLDAALQ
DEVAASEGFLKQPAGKEYAFAGPSVKDKKYFGDGTGVGLRKDDTELKAAFDKALTELRQDGTYDKMAKKYFDFNVYGD