Crystal structure of Deoxyuridine 5'-triphosphate nucleotidohydrolase from Legionella pneumophila Philadelphia 1 in complex with dUMP (Deoxyuridine 5'-monophosphate)

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Deoxyuridine 5'-triphosphate nucleotidohydrolase

MAHHHHHHMHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVAPQQTVLLPTGIAIYIADPKLAAVILPRS
GLGHKNGIVLGNLVGLIDSDYQGELKISCWNRSQEHFTVNPGDRIAQLVFIPVVQASFEVVNEFTESSRGEGGFGSSGRY

Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Molecular Dimensions Morpheus screen, condition C4: 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD 30mM each sodium nitrate, disodium hydrogen phosphate, ammonium sulfate: 100mM MES/imidazole pH 6.5: LepnA.01206.a.B1.PS38438 at 24.33mg/ml: 2h soak with 5mM MgCl2 + dUTP with was converted to dUMP: cryo: direct: EG in two steps: tray 301633a1, puck PXP9-8

Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases

SSGCID, Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, HYDROLASE

MHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVAPQQTVLLPTGIAIYIADPKLAAVILPRSGLGHKNGI
VLGNLVGLIDSDYQGELKISCWNRSQEHFTVNPGDRIAQLVFIPVVQASFEVVNEFTESSRGEGGFGSSGRY