Cryo-EM structures of SADS-CoV spike glycoproteins

Cryo-EM structures of HKU2 and SADS-CoV spike glycoproteins provide insights into coronavirus evolution.

Swine acute diarrhea syndrome coronavirus

MKLFTVFTLLASIRVLYGCESVDFNLFNTIFSTHRGLSNTTSVITGAYPSTNKSDWSCNTRTGHLSGSGFGIGLYVQTPR
EQYQYDGSGAGGYTIAVSPIHVTNLTWELWIHRKWGVNSVVTVRLCRWWQFMSFNSTSHAADAGPTNAFECLINGSYPTH
RNTGYMFGVTWYNDLVRIVFPPTVLEMQLDGLQWERVQFNSPVNAGHATRFNVVKDISTVLVETNSGGSVFRYSYCADGF
VNGLQCKLRLFDIPPGVYSNSEVEYPTALYTVVHNMSACPERPDSYCGSNSCPFKRAVFSNCIVNYTTWVNPDQRDFQHL
ILSNGKFNPFTECNGLNRIVDGCVPGFVLRVGRGKAVNRTIVTPYLKPYECFGWSWNDNQDSIYDWWIADFVSTGAFVCE
SNPEAPKTGVCVTYTVEKVTFQGVLYESNFTFAQYYNLLYVGSQLRYVRILGKVYEVSSCFEASYDVLYRNNQSFGLLYR
SFDCNQLHIKSARFVDRLLPSHNGTATVLGCLFNASYAPNDTMVNCTNPLGDGFCADLLGNVAVRRMTFEKHDTTYVAPV
TNERYTEMPLDHQLILTEQFLQTTMPKFSVSCETYICDVSKACKNLLFRYGGFCQKVEADIRGAGILLDGDVSSLYSTIA
AKTSSVVPTTDRFNVSQFFLPKTQSSANKYESRSAIEDLLFSKIETTGPGFYGDYYNCKKNAIQDLTCAQYHNGILVIPP
IMDAETLGMYGGIAAASVTLGIFGGQAGMATWSVAMAGRLNALGVVQNALVDDVNKLANGFNQLTASVSKLALTTSSALQ
AIQAVVNQNAAQVESLVSGITENFGAISTNFKVISQRLDKLEADVQMDRLINGRMNVLQLFVTNYKLKIAELRNTHRYVQ
SLINECVYAQSLRNGFCGQGLHVLSLMQNAPSGIMFFHYSLIPNNTITVKTTPGLCESDELGSKCIVAKDGVLVSANLSY
WQWSPRNLYKPENLTFANVIAVSRGANYTTLNKTFDIPELNSTFPIEEEFREYFQNMSSELQVLKNLTADMSKLNISAEI
QLINEIAHNVSNMRVEVEKFQRYVNYVKLEVLFQGPGGGSGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGWSHPQFEK

Cryo-EM structures of HKU2 and SADS-CoV spike glycoproteins and insights into coronavirus evolution

The L1069-G1112 is expression tag for trimerization and W1113-K1120 is strep tag used for purification.

MKLFTVFTLLASIRVLYGCESVDFNLFNTIFSTHRGLSNTTSVITGAYPSTNKSDWSCNTRTGHLSGSGFGIGLYVQTPR
EQYQYDGSGAGGYTIAVSPIHVTNLTWELWIHRKWGVNSVVTVRLCRWWQFMSFNSTSHAADAGPTNAFECLINGSYPTH
RNTGYMFGVTWYNDLVRIVFPPTVLEMQLDGLQWERVQFNSPVNAGHATRFNVVKDISTVLVETNSGGSVFRYSYCADGF
VNGLQCKLRLFDIPPGVYSNSEVEYPTALYTVVHNMSACPERPDSYCGSNSCPFKRAVFSNCIVNYTTWVNPDQRDFQHL
ILSNGKFNPFTECNGLNRIVDGCVPGFVLRVGRGKAVNRTIVTPYLKPYECFGWSWNDNQDSIYDWWIADFVSTGAFVCE
SNPEAPKTGVCVTYTVEKVTFQGVLYESNFTFAQYYNLLYVGSQLRYVRILGKVYEVSSCFEASYDVLYRNNQSFGLLYR
SFDCNQLHIKSARFVDRLLPSHNGTATVLGCLFNASYAPNDTMVNCTNPLGDGFCADLLGNVAVRRMTFEKHDTTYVAPV
TNERYTEMPLDHQLILTEQFLQTTMPKFSVSCETYICDVSKACKNLLFRYGGFCQKVEADIRGAGILLDGDVSSLYSTIA
AKTSSVVPTTDRFNVSQFFLPKTQSSANKYESRSAIEDLLFSKIETTGPGFYGDYYNCKKNAIQDLTCAQYHNGILVIPP
IMDAETLGMYGGIAAASVTLGIFGGQAGMATWSVAMAGRLNALGVVQNALVDDVNKLANGFNQLTASVSKLALTTSSALQ
AIQAVVNQNAAQVESLVSGITENFGAISTNFKVISQRLDKLEADVQMDRLINGRMNVLQLFVTNYKLKIAELRNTHRYVQ
SLINECVYAQSLRNGFCGQGLHVLSLMQNAPSGIMFFHYSLIPNNTITVKTTPGLCESDELGSKCIVAKDGVLVSANLSY
WQWSPRNLYKPENLTFANVIAVSRGANYTTLNKTFDIPELNSTFPIEEEFREYFQNMSSELQVLKNLTADMSKLNISAEI
QLINEIAHNVSNMRVEVEKFQRYVNYVK