Crystal structure of GPR52 ligand free form with rubredoxin fusion

(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

Structural basis of ligand recognition and self-activation of orphan GPR52.

Chimera of G-protein coupled receptor 52 and Rubredoxin

GGIVNVSERHSCPLGFGHYSVVDVCIFETVVIVLLTFLIIAGNLTVIFVFHCAPLLHHYTTSYFIQTMAYADLFVGVSCL
VPTLSLLHYSTGVHESLTCQVFGYIISVLKSVSMWCLACISVDRYLAITKPLSYNQLVTPCRLRICIILIWIYSCLIFLP
SFFGWGKPGYHGDIFEWCATSWLTSAYFTGFIVCLLYAPAAFVVCFTYFHIFKICRQHTKMKKYTCTVCGYIYNPEDGDP
DNGVNPGTDFKDIPDDWVCPLCGVGKDQFEEVEEYLMVLFRITSVFYMLQLPYIIYFLLESSRVLDNPTLSFLTTWLAIS
NSFCNPVIYALSDSTFRLGLRRLSETMCTSCMEFLEVLFQGPHHHHHHHHHH

0.08-0.1 M magnesium sulphate, 0.1 M sodium cacodylate trihydrate pH 6.2, and 28-31% PEG300

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Authors aniso-corrected the observed data by STARANISO which remove weak reflections.

AUTHORS STATE THAT THE BIOLOGICAL UNIT IS UNKNOWN.

Human GPR52 receptor, Class A, orphan GPCR, membrane protein, apo form, rubredoxin, LCP

GIVNVSERHSCPLGFGHYSVVDVCIFETVVIVLLTFLIIAGNLTVIFVFHCAPLLHHYTTSYFIQTMAYADLFVGVSCLV
PTLSLLHYSTGVHESLTCQVFGYIISVLKSVSMACLACISVDRYLAITKPLSYNQLVTPCRLRICIILIWIYSCLIFLPS
FFGWGKPGYHGDIFEWCATSWLTSAYFTGFIVCLLYAPAAFVVCFTYFHIFKICRQHTK