Cytosine epigenetic modification modulates the formation of an unprecedented G4 structure in the WNT1 promoter.
DNA (5'-D(*GP*GP*GP*TP*CP*AP*CP*CP*GP*GP*GP*CP*AP*GP*TP*GP*GP*GP*CP*GP*GP*G)-3')
(DG)(DG)(DG)(DT)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DC)(DA)(DG)(DT)(DG)(DG)(DG)(DC)(DG) (DG)(DG)
1 mM WT22m1, 90 mM potassium chloride, 10 mM potassium phosphate, 100 uM DSS, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
By using native gel electrophoresis, CD and NMR spectroscopy, DNA G-qaudruplex can be fold in the buffer condition with potassium ion