X-ray Structure of the proton-pumping cytochrome aa3-600 menaquinol oxidase from Bacillus subtilis

Structure of the cytochromeaa3-600 heme-copper menaquinol oxidase bound to inhibitor HQNO shows TM0 is part of the quinol binding site.

Cytochrome aa3 quinol oxidase subunit I,Cytochrome aa3-600 quinol oxidase subunit I

Cytochrome aa3-600 quinol oxidase subunit III,Quinol oxidase subunit 3

Cytochrome aa3-600 quinol oxidase subunit IV,Quinol oxidase subunit 4

MKFKWDEFFVTGDPLILGAQVSIALSTIAIIFVLTYFKKWKWLWSEWITTVDHKKLGIMYIISAVIMLFRGGVDGLMMRA
QLALPNNSFLDSNHYNEIFTTHGTIMIIFMAMPFLIGLINVVVPLQIGARDVAFPYLNNLSFWTFFVGAMLFNISFVIGG
SPNAGWTSYMPLASNDMSPGPGENYYLLGLQIAGIGTLMTGINFMVTILKMRTKGMTLMRMPMFTWTTLITMVIIVFAFP
VLTVALALLSFDRLFGAHFFTLEAGGMPMLWANLFWIWGHPEVYIVILPAFGIFSEIISSFARKQLFGYKAMVGSIIAIS
VLSFLVWTHHFFTMGNSASVNSFFSITTMAISIPTGVKIFNWLFTMYKGRISFTTPMLWALAFIPNFVIGGVTGVMLAMA
AADYQYHNTYFLVSHFHYVLIAGTVFACFAGFIFWYPKMFGHKLNERIGKWFFWIFMIGFNICFFPQYFLGLQGMPRRIY
TYGPNDGWTTLNFISTVGAFMMGVGFLILCYNIYYSFRYSTREISGDSWGVGRTLDWATSSAIPPHYNFAVLPEVKSQDA
FLHMKEEKTELYPESKFKKIHMPSNSGRPFFMSVAFGLAGFGLVFEWYWMGVVGLIGVLLCMVLRSFEYDNGYYISVDEI
KETERKISEHHHHHH

CSNASVLDPKGPVAEQQSDLILLSIGFMLFIVGVVFVLFTIILVKYRDRKGKDNGSYNPEIHGNTFLEVVWTVIPILIVI
ALSVPTVQTIYSLEKAPEATKDKEPLVVYATSVDWKWVFSYPEQDIETVNYLNIPVDRPILFKISSADSMASLWIPQLGG
QKYAMAGMLMDQYLQADKVGTYEGRNANFTGEHFADQEFDVNAVTEKDFNSWVKKTQNEAPKLTKEKYDELMLPENVDEL
TFSSTHLKYVDHGQDAEYAMEARKRLGYQAVSPHSKTDPFENVKKNEFKKSDDTEE

MEHAEHGNSNAPMEYQSETGRLNILGFWIFLGAEIVLFSTLFATFFVLKNRTAGGVLPDELFEVNLVMIMTFLLLISSFT
CGIAVHEMRRGSLKGVVIWTIITLLLGAGFVGCEINEFVHYVHEGAALSTSAFWSGFFVLLGTHGTHVTIGIFWITGILI
QLKKRGLTPQTSSKIFISSLYWHFLDVVWIFIFTGVYLMGLGGL

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)FGFAF
IQAALQLLMFMHMTESENGTIQVGNTLFGFFGAIVIVLGSIWIFAAHYHHGDHMDGNPPGGAEHSEHSGHNE

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGFAFIQAALQLLMFMHMTESENGTIQVGNTLF
GFFGAIVIVLGSIWIFAAHYHHGDHMDGNPPGGAEHSEHSGHNE

0.1 M Calcium chloride, 0.1M Tris pH 6.3, 13% PEG 2000 MME

Authors know the sequence of chain D/H, but they are not sure of the alignment for first 22 residues in the coordinates. The residue numbers 0-21 in the coordinates may be meaningless. The correct sequence is MANKSAEHSHFPWKHIVGFILSIVLTLLALWVAVYTDLSSSAKLWII (UNP P34959)

Menaquinol oxidase, Complex, Proton pumping, inhibitor, OXIDOREDUCTASE

MKFKWDEFFVTGDPLILGAQVSIALSTIAIIFVLTYFKKWKWLWSEWITTVDHKKLGIMYIISAVIMLFRGGVDGLMMRA
QLALPNNSFLDSNHYNEIFTTHGTIMIIFMAMPFLIGLINVVVPLQIGARDVAFPYLNNLSFWTFFVGAMLFNISFVIGG
SPNAGWTSYMPLASNDMSPGPGENYYLLGLQIAGIGTLMTGINFMVTILKMRTKGMTLMRMPMFTWTTLITMVIIVFAFP
VLTVALALLSFDRLFGAHFFTLEAGGMPMLWANLFWIWGHPEVYIVILPAFGIFSEIISSFARKQLFGYKAMVGSIIAIS
VLSFLVWTHHFFTMGNSASVNSFFSITTMAISIPTGVKIFNWLFTMYKGRISFTTPMLWALAFIPNFVIGGVTGVMLAMA
AADYQYHNTYFLVSHFHYVLIAGTVFACFAGFIFWYPKMFGHKLNERIGKWFFWIFMIGFNICFFPQYFLGLQGMPRRIY
TYGPNDGWTTLNFISTVGAFMMGVGFLILCYNIYYSFRYSTREISGDSWGVGRTLDWATSSAIPPHYNFAVLPEVKSQDA
FLHMKEEKTELYPESKFKKIHMPSNSGRPFFMSVAFGLAGFGLVFEWYWMGVVGLIGVLLCMVLRSFEYDNGYYISVDEI
KETERKISE