Crystal structure of SucA with glycolaldehyde-1-13C from Vibrio vulnificus

Understanding the molecular properties of the E1 subunit (SucA) of alpha-ketoglutarate dehydrogenase complex from Vibrio vulnificus for the enantioselective ligation of acetaldehydes into (R)-acetoin.

Oxoglutarate dehydrogenase (Succinyl-transferring), E1 component

MGSSHHHHHHDYDIPTTENLYFQGGGGGGDVDAKQVKVLQLINAYRFRGHEAAELDPLGLWQRPTVAELDPAFHNLTEDD
FEETFNVGSFAVGQETMPLKDIYTALKKTYCGSIGAEYMHMTDTEQKRWIQQRLESVVGQPSFDKDEKRTFLAELTAAEG
LERYLGAKFPGAKRFSLEGGDAMIPMMKELIRHAGRSGMREVVIGMAHRGRLNMLVNVLGKKPQDLFDEFAGKHGESWGT
GDVKYHQGFSADFATPGGDVHLALAFNPSHLEIVNPVVMGSVRARQDRLGDDDGSKVLPITIHGDSAIAGQGVVAETFNM
SQARGFCVGGTVRVVVNNQVGFTTSNPRDTRSTMYCTDIAKMVQAPIFHVNADDPEAVAFVTRIALDYRNEFKRDVVIDL
VCYRRHGHNEADEPNATQPLMYQKIKKHPTPRKLYADVLIDRNECDIETATQMVNEYRDALDHGEVVVKEWRPMALHSVD
WSPYLGHEWDTPWSNTYDKQRLVELGKRLCQYPESHTLHSRVSKLYNDRTAMTNGEKELDWGMAETLAYATLVDDGKRIR
ISGQDSGRGTFFHRHAVLHNQNDASTYVPLANIHDKQGPFEVFDSVLSEEAVLAFEYGYATAEPSGLTLWEAQFGDFANG
AQVVIDQFISSGEQKWARLCGLTMLLPHGYEGQGPEHSSARLERYLQLCAEQNMQVVVPSTPAQVYHMIRRQVVRPMRRP
LIVMSPKSLLRHPLCTSSLDDLANGTFMPAIPEIDELDPAKVKRVVFCSGKVYFDLLEQRRNNEQDDVAIVRIEQLYPFP
MDDVKAAIAPYVNVEDFVWCQEEPQNQGAWYCSQHNFRAAIPAGTELKYAGRPASASPAVGYMSVHLKQQKALIDDALNV
NEKTSD

220 mM calcium acetate, 100mM 2-(Bis(2-hydroxyethyl)amino)acetic acid (BICINE), 50% (v/v) PEG300, 0.1 mM ThDP, 0.1 mM MgCl2

(chain A and (resid 87 through 288 or resid 294 through 935))
(chain B and (resid 87 through 288 or resid 294 through 935))
(chain C and (resid 87 through 288 or resid 294 through 935))

DVDAKQVKVLQLINAYRFRGHEAAELDPLGLWQRPTVAELDPAFHNLTEDDFEETFNVGSFAVGQETMPLKDIYTALKKT
YCGSIGAEYMHMTDTEQKRWIQQRLESVVGQPSFDKDEKRTFLAELTAAEGLERYLGAKFPGAKRFSLEGGDAMIPMMKE
LIRHAGRSGMREVVIGMAHRGRLNMLVNVLGKKPQDLFDEFAGKHGESWGTGDVKYHQGFSADFATPGGDVHLALAFNPS
HLEIVNPVVMGSVRARQDRLGDDDGSKVLPITIHGDSAIAGQGVVAETFNMSQARGFCVGGTVRVVVNNQVGFTTSNPRD
TRSTMYCTDIAKMVQAPIFHVNADDPEAVAFVTRIALDYRNEFKRDVVIDLVCYRRHGHNEADEPNATQPLMYQKIKKHP
TPRKLYADVLIDRNECDIETATQMVNEYRDALDHGEVVVKEWRPMALHSVDWSPYLGHEWDTPWSNTYDKQRLVELGKRL
CQYPESHTLHSRVSKLYNDRTAMTNGEKELDWGMAETLAYATLVDDGKRIRISGQDSGRGTFFHRHAVLHNQNDASTYVP
LANIHDKQGPFEVFDSVLSEEAVLAFEYGYATAEPSGLTLWEAQFGDFANGAQVVIDQFISSGEQKWARLCGLTMLLPHG
YEGQGPEHSSARLERYLQLCAEQNMQVVVPSTPAQVYHMIRRQVVRPMRRPLIVMSPKSLLRHPLCTSSLDDLANGTFMP
AIPEIDELDPAKVKRVVFCSGKVYFDLLEQRRNNEQDDVAIVRIEQLYPFPMDDVKAAIAPYVNVEDFVWCQEEPQNQGA
WYCSQHNFRAAIPAGTELKYAGRPASASPAVGYMSVHLKQQKALIDDALNVNEKTSD