Cryo-EM structure of Thermococcus kodakarensis RNA polymerase

Direct binding of TFE alpha opens DNA binding cleft of RNA polymerase.

DNA-directed RNA polymerase subunit beta

TFE,TFIIE subunit alpha homolog,Transcription initiation factor TFIIE

MQSMKKVIGSIEFGILSPQEIRKMSAAEITVPDTYDDDGYPIEGGLMDKRLGVIDPGLRCETCGARAGECPGHFGHIELA
RPVIHVGFAKTIHRVLESTCRECGRIKLTDEEIEEYMQKFEVMGDRKGAVDKLIKEIHKKAKERMVCPHCGAPQFPIKFE
RPTIYWELRKDEEGNEYKHRMMPSEVRDRLEKIPDKDLPLLGLHPEKSRPEWMVLTVLPVPPVTMRPSITLESGIRAEDD
LTHKLVDIIRINNRLKSNIEAGAPQLIIEDLWDLLQYHVTTYINNETSGVPPAKHKSGRPLKTLAQRLKGKEGRFRGNLS
GKRVNFSARTVISPDPMISINEVGVPLAVAMELTVPEKVTEFNYEKLKQRVLNGPEKYPGANYVIDPEGRRIRLMESNRE
LIAEKLDIGWTVERHLEDGDVVLFNRQPSLHRMSIMAHRVRVMPYRTFRLNLPVCPPYNADFDGDEMNLHVPQTEEAQAE
AKILMEVQNHIISPRYGGPLIAGIQDHISGGYLLTREGAYFTRYEVEQMLMFAGMDVNELPEPDKYENGEPLWSGKTIFS
LLLPDDLTIWYRNKLCDEPERCEALEKLIEEKLIPDPEEVRKLAYDGFVYIQNGKLLSGAVDKKAYGREDGKLLDIIVRE
YGVERARQFLDQVTKLTIWVITHKGFTTAIDDEDLPQEAIDRIHEIIREAEEKVQRLIEAYKRGELEPLPGKTLEETLES
KIMAVLAEARDNAGKVAERYLGMNNHAVIMAKTGARGKILNITQMAAMLGQQSIRGKRLYRGYRGRVLTHFKPGDLGARA
RGFVTNSYKSGLTPQEYFFHAMGGREGLVDTAVRTAQSGYMQRRLINALQDLKVDYDGTVRDPTGIIVQFKYGEDGVDPM
KSWQGKTVDVDRVIVRTLLKMRGGGE

MSRGVTVTEPTLTPDELWLVMESYWKEKGFVRQHLDSYNAFIDHGLQDVVNEFGEVVPDIPNFKVKFGKIRIGQPEFQEA
QGQRRPLYPMDARIRNLTYSAPLYLEMIPVVNGIEQEPVEVRIGELPIMLKSKACRLYGLSDEELIKLGEDPKDPGGYFI
INGSERVIVSIEDLAPNKTLVERDERQNKVVAKVFSYRHGYRALITVERKKDGILYVTIPNVPKPVKFVYVMRALGLLTD
KEIVEAVSDDPRIQQVLFDNLEDASDISTQEEALDYIGRLALPGQPKEYRLRRAEHIIDNNLLPHMGVDPENRRAKAYYL
GMMALKVLELSLGLRGEDDKDHYANKRLKLAGDLLKDLFRVAFGQLVKDMQYQMTKTYQRKGERYTFENIQRFVRNSIRP
DVLSERIEHALATGSWPGGRTGVSQLLDRTNYMSTLSHLRRVTSPLSRDQPHFEARDLHGTHWGRICPTETPEGPNCGLV
KNLALMSQITTGIPEREVREYLMKMGVVPIEERRPAPGLYRVYLNGVLIGTVEDGRKLVERIRADRRAGKISDVINVALY
EDEEVKEVYINSDDGRVRRPLIVVENGKPKLTREHVEGIKNGTLTWSDLIRMGVIEYLDAEEEENAYVATWPWEVTEEHT
HLELMPAAILGIPASLVPYPEHNAAPRNTYGAGMAKQSLGLGWANFRIRVDTRGHLMHYPQVPLVNSRIMKAVGFEDRPA
GQNFVVAVLSYHGYNMEDAVIINKASIERGLARSTFFRTYEAEEKRYLGGQKDNFEVPSPNIQGYLGEKYYRHLDEDGLI
FPESKVEGKDVLVGRTSPPRFIEEQSSLGSMVLQGRRETSVTVRPSEKGVVDKVIVTETGDGTKLVKVTVRDLRIPELGD
KFASRHGQKGVIGLIVPQEDMPWTESGIVPDLIVNPHGIPSRMTVGQLIEAIGGKVASLTGRRVDGTAFIGEPEEKLRKE
LEELGFKHSGREIMYDGITGRRLEADIFIGVIYYQRLHHMVADKMHARSRGPVQVLTKQPTEGRAREGGLRFGEMERDVL
IGHGAAMLLIERLLEESDKTEVWVCESCGHLALEDKRRGKVYCPVCGEDERISKVEMSYAFKLLLDELKAMVIRPSLRLK
DRV

MVAEKTIKSMVSKAELPDNIKEELYAKLIEYNEKYKLKKDEIQAIIDETVREYQKALIEPGEAVGTVAAQSIGEPSTQMT
LNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPIMTVYLDEEHRYDRDKALEVARRIEGTTLENLAREETIDILNMEYV
VEIDPERLEKAGLDMEKVVRKLTGSFKSAEFEAEGYTLVVRPKKVTKLSDLRKIAEKVKKHRLKGLSGVGKTIIRKEGDE
YVIYTEGSNFKQVLKVPGVDPTRTRTNNIWEIAEVLGIEAARNAIIDEIVSTMREQGLEVDVRHIMLVADMMTLDGVIRP
IGRHGIVGEKASVLARAAFEITTQHLFAAAERGEVDPLNGVVENVLIGQPVPVGTGIVKLAMSLPLRPKRE

MVEFKILEKRPDSIKFIVSGVDVPFANALRRTILSEVPTFAVDEVEFLENDSALFDEIIAHRLAMIPLTTPHERFSLDAL
ELDDYTVTLSLEAEGPGMVYSGDLKSSDGDVKPANPNIPIVKLAEGQRLTFNAYARLGRGKDHAKWQPGFVYYKYLTKIH
VSKDVPDWEELKELAERRGLPVEESDEEIVITTIKAFYLPRKFEEHMGKGIREEIVPGSFVFTVETNGELPVEEIVSIAL
KILMRKSDRFINELHKLAD

MYKLLKVKDVVRIPPRMFTMDPKEAAKIVLRETYEGIYDRDEGVVLAILDVEEISEGVIVPGDGATYHEAIFNVLVWEPR
NQEVVEGEVVEMMPYGAFIRIGPMDGLVHISQLMDDYVVFDEKNRQFIGKETNRVLKLGDYVRARIIGVSVKSRVIRENK
INMTMRQPGLGKFEWIEKEKKKAKEESKGE

MIGRKKLEEHYITIAEAKELLERRHAEGLAENPEEPMFYEARVSLEHAERFAKLKPEQARELKEKLMGLFDWINERIAAK
LVDILPEDYLDIRVIFAKEEYMPTPEEAEEIIKVIDEYRPLE

MAAKKEFNIFDHVLVPEHRILSEEEKEELLKKYRIRISQLPQIKASDPAVVALGAKPGDVIEIKRKSPTAGYYYYYRLVV
ED

MRIEVIRREENLLEFYLEGEDHTFANLLTETLHENEHVTFAGYTIEHPITMARKPRFKVVTDGKITPEKALEEAAQKIFD
RAREVLEAWKAAIE

MAKRKNKELLEIAQEIGGEEAVEVVKALEKMKEATDEELAEATGIRVNTVRRILYMLNDEGLADFKRIRDPETGWYYYYW
RLETKKLPEIIRSRKMAELKKLKEMLEEETSEIYYWCGTEGHPKLTFDEAMEYEFQCPICGKMLMQYDNTHIIEELKRRI
EELEIELGLKKKPRKTSKKKKSRSE

Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

Ministry of Science, ICT and Future Planning

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

MIGRKKLEEHYITIAEAKELLERRHAEGLAENPEEPMFYEARVSLEHAERFAKLKPEQARELKEKLMGLFDWINERIAAK
LVDILPEDYLDIRVIFAKEEYMPTPEEAEEIIKV