Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis

Colonization factor antigen I chaperone CfaA

ANFMIYPISKDLKNGNSELVRVYSKSKEIQYIKIYTKKIINPGTTEEYEVDIPNWDGGLVVTPQKVILPAGASKSIRLTQ
FKIPKKEEVYRVYFEAVKPDSKENVIDNKKLTIEISINIIYAALIRSLPSEQNISLNISRNAKKNIIIYNNGNVRAGVKD
IYFCKSSNIDDNCVKKAYNKNIYPEKSFDTLVNNNFSYVFIKLNHEGIEKEQGLIQLKVPA

HHHHDKNPGSENMTNTIGPHDRGGSSPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETN
ITLQFTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAGELNKLPFGGVWNAVLKLNV
KRRYDTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVDLNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNS
KSDGKFYLKKINDDSKELVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLDAKVKN
PEAGQYMGNIKITFTPSSQTL

2%PEG8000, 0.1M imidazole malate, 1.04M Li2SO4, 0.01M GSSG-GSH

Colonization factor antigen I chaperone CfaA, CFA/I fimbrial subunit E

Enterotoxigenic Escherichia coli, chaperone-usher fimbriae, periplasmic chaperone, adhesive subunit, binding motif, CHAPERONE

ANFMIYPISKDLKNGNSELVRVYSKSKEIQYIKIYTKKIINPGTTEEYEVDIPNWDGGLVVTPQKVILPAGASKSIRLTQ
FKIPKKEEVYRVYFEAVKPDSKENVIDNKKLTTELSVNIIYAALIRSLPSEQNISLNISRNAKKNIIIYNNGNVRAGVKD
IYFCKSSNIDDNCVKKAYNKNIYPEKSFDTLVNNNFSYVFIKLNHEGIEKEQGLIQLKVP

DKNPGSENMTNTIGPHDRGGSSPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETNITLQ
FTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAGELNKLPFGGVWNAVLKLNVKRRY
DTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVDLNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNSKSDG
KFYLKKINDDSKELVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLDAKVKNPEAG
QYMGNIKITFTPSSQTL