Structure of light-state marine bacterial chloride importer, NM-R3, with CW laser (ND-10%) at 95K.

Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family.

MASMTGGQQMGRDPNSMKNIESLFDYSAGQFEFIDHLLTMGVGVHFAALIFFLVVSQFVAPKYRIATALSCIVMVSAGLI
LNSQAVMWTDAYAYVDGSYQLQDLTFSNGYRYVNWMATIPCLLLQLLIVLNLKGKELFSTATWLILAAWGMIITGYVGQL
YEVDDIAQLMIWGAVSTAFFVVMNWIVGTKIFKNRATMLGGTDSTITKVFWLMMFAWTLYPIAYLVPAFMNNADGVVLRQ
LLFTIADISSKVIYGLMITYIAIQQSAAAGYVPAQQALGRIGMDSKAALEHHHHHH

30% polyethylene glycol dimethyl ether 500, 0.15 M sodium chloride, 0.15M calcium chloride, and 0.1 M MES (pH 6.0) buffer

Photo-excitation, Chloride pump, Retinal, MEMBRANE PROTEIN

MKNIESLFDYSAGQFEFIDHLLTMGVGVHFAALIFFLVVSQFVAPKYRIATALSCIVMVSAGLILNSQAVMWTDAYAYVD
GSYQLQDLTFSNGYRYVNWMATIPCLLLQLLIVLNLKGKELFSTATWLILAAWGMIITGYVGQLYEVDDIAQLMIWGAVS
TAFFVVMNWIVGTKIFKNRATMLGGTDSTITKVFWLMMFAWTLYPIAYLVPAFMNNADGVVLRQLLFTIADISSKVIYGL
MITYIAIQQSAAAGYVPAQQALGRIGMDSKAA