Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5

1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase.

Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) dodecamer in complex with Mg2+, NADH, and CPD at pH7.5

Acetohydroxy-acid isomeroreductase 2,Alpha-keto-beta-hydroxylacyl reductoisomerase 2

MDKTVLDANLDPLKGKTIGVIGYGNQGRVQATIMRENGLNVIVGNVKDKYYELAKKEGFEVYEIDEAVRRSDVALLLIPD
EVMKEVYEKKIAPVLQGKKEFVLDFASGYNVAFGLIRPPKSVDTIMVAPRMVGEGIMDLHKQGKGYPVLLGVKQDASGKA
WDYAKAIAKGIGAIPGGIAVISSFEEEALLDLMSEHTWVPILFGAIKACYDIAVKEYGVSPEAALLEFYASGELAEIARL
IAEEGIFNQMVHHSTTSQYGTLTRMFKYYDVVRRIVENEAKYIWDGSFAKEWSLEQQAGYPVFYRLWELATQSEMAKAEK
ELYKLLGRKVKND

Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Ministry of Science and Technology (Taiwan)

Bi-specific, Thermostable, Reductoisomerase, Magnesium-dependent, Dodecamer, Knotted protein, ISOMERASE