Crystal Structure Analysis of the Glycotransferase of kitacinnamycin

Genome mining and biosynthesis of kitacinnamycins as a STING activator.

GSHMPRPGHIAMVSVPSRGHLHPSLELIRELVARGHRVTYANDPSVAAAVTETGAELVPYTSALPSTDTTEGRVTDQIAQ
MDVFLDDAVGMLPQLRAAYEEDRPDVFLYDVLAYPARVLAMNWGIPSIQISPTWVMPEKYRERMAPVVEQLKQDPRGAAH
YRRFDAWLEDSGVPGIDAGDLVNLPERSLVLVPRFLQPDADDVDEKRFTFIGPCLGRRAHQGDWKRPAGAEKVALVSLGS
HLTNQLPFYETCVEVFAALPDWHLVLQIGRHVDAGELGELPPNVEVHNWVPQLAVLEQADVFVTHGGMGGIQEGLFSGVP
MVVAPQANDQPANAESVVGLGIARRIDIATVTPDRLRAAVVELASDPAVAERLSGLRRELRAHGGTMRAADLIERQLPA

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

1.6 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane

(chain A and (resid 2 through 62 or resid 73 through 136 or (resid 137 and (name N or name CA or name C or name O or name CB )) or resid 138 through 139 or (resid 140 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 149 or (resid 150 and (name N or name CA or name C or name O or name CB )) or resid 151 through 214 or (resid 215 through 216 and (name N or name CA or name C or name O or name CB )) or resid 217 through 221 or (resid 222 through 223 and (name N or name CA or name C or name O or name CB )) or resid 224 through 238 or (resid 239 and (name N or name CA or name C or name O or name CB )) or resid 240 through 301 or (resid 302 and (name N or name CA or name C or name O or name CB )) or resid 303 through 395))

(chain B and (resid 2 through 62 or resid 73 through 136 or (resid 137 and (name N or name CA or name C or name O or name CB )) or resid 138 through 139 or (resid 140 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 221 or (resid 222 through 223 and (name N or name CA or name C or name O or name CB )) or resid 224 through 350 or (resid 351 and (name N or name CA or name C or name O or name CB )) or resid 352 through 395))

(chain C and (resid 2 through 14 or (resid 15 and (name N or name CA or name C or name O or name CB )) or resid 16 through 62 or resid 73 through 140 or (resid 141 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 149 or (resid 150 and (name N or name CA or name C or name O or name CB )) or resid 151 through 214 or (resid 215 through 216 and (name N or name CA or name C or name O or name CB )) or resid 217 through 238 or (resid 239 and (name N or name CA or name C or name O or name CB )) or resid 240 through 246 or (resid 247 and (name N or name CA or name C or name O or name CB )) or resid 248 through 301 or (resid 302 and (name N or name CA or name C or name O or name CB )) or resid 303 through 350 or (resid 351 and (name N or name CA or name C or name O or name CB )) or resid 352 through 395))

(chain D and (resid 2 through 14 or (resid 15 and (name N or name CA or name C or name O or name CB )) or resid 16 through 62 or resid 73 through 76 or (resid 77 and (name N or name CA or name C or name O or name CB )) or resid 78 through 136 or (resid 137 and (name N or name CA or name C or name O or name CB )) or resid 138 through 139 or (resid 140 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 149 or (resid 150 and (name N or name CA or name C or name O or name CB )) or resid 151 through 214 or (resid 215 through 216 and (name N or name CA or name C or name O or name CB )) or resid 217 through 221 or (resid 222 through 223 and (name N or name CA or name C or name O or name CB )) or resid 224 through 238 or (resid 239 and (name N or name CA or name C or name O or name CB )) or resid 240 through 246 or (resid 247 and (name N or name CA or name C or name O or name CB )) or resid 248 through 301 or (resid 302 and (name N or name CA or name C or name O or name CB )) or resid 303 through 350 or (resid 351 and (name N or name CA or name C or name O or name CB )) or resid 352 through 395))