Solution structure of translationally controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica
Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity.
MIVYKDVISGDEVVSDALKITPVMEGGEEVPGLFEVDSAMVAVGGGDIDIGCGNAFGGAGDDEGADDATQKENNVSGPSS FAYTAMPFSSKGEFKSWVKDYVRNVRQALKGSGVAVEDIKKFMEEAPTFVKWLVDKYDDLEFFMSKSMNPDAGLIFSYYK EGAHCPTFVYVKSGYKVVKFLEHHHHHH
Ministry of Science and Technology (China)
1 mM [U-13C; U-15N] NoTCTP, 50 mM sodium phosphate, 200 mM potassium chloride, 5 mM DTT, 5 mM EDTA, 0.02 % w/v sodium azide, 0.01 % w/v DSS, 90% H2O/10% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
translation, TCTP, eEF1B-binding protein