The ligand binding domain of Mlp24 with asparagine

Calcium Ions Modulate Amino Acid Sensing of the Chemoreceptor Mlp24 ofVibrio cholerae.

GPLGSVREEIESLVQDSLMEMVKGVKNTIESDLASKKGLAQSTTEILQLDPTNKAFAKSVLESPNLKGSFLAIGLGYESD
ATVVENDDGWEPNADYDPRKRPWYVDAKRERKLVVTEPYVDISTKKIIISIGTPVYQQSNFVGAMFYDVELTQLAQLVNS
VNLFDAGYLFITTKDGVTIAHPNAENNGEKFSQFLPNVDLKEGTQRIELDGKYYLVKFAQVPSESWYIGAVVDESIAFAM
VDDLRHSSLIHHHHHH

0.1M MES pH6.0, 50% (v/v) PEG 400, 0.1M calcium acetate

Japan Society for the Promotion of Science

Ministry of Education, Culture, Sports, Science and Technology (Japan)

CHEMORECEPTOR, LIGAND COMPLEX, MCP-LIKE PROTEIN, PAS-LIKE DOMAIN, SIGNALING PROTEIN

VREEIESLVQDSLMEMVKGVKNTIESDLASKKGLAQSTTEILQLDPTNKAFAKSVLESPNLKGSFLAIGLGYESDATVVE
NDDGWEPNADYDPRKRPWYVDAKRERKLVVTEPYVDISTKKIIISIGTPVYQQSNFVGAMFYDVELTQLAQLVNSVNLFD
AGYLFITTKDGVTIAHPNAENNGEKFSQFLPNVDLKEGTQRIELDGKYYLVKFAQVPSESWYIGAVVDESIAFAMVDDLR
HSSLI