The ligand binding domain of Mlp24 with glycine

Calcium Ions Modulate Amino Acid Sensing of the Chemoreceptor Mlp24 ofVibrio cholerae.

GPLGSVREEIESLVQDSLMEMVKGVKNTIESDLASKKGLAQSTTEILQLDPTNKAFAKSVLESPNLKGSFLAIGLGYESD
ATVVENDDGWEPNADYDPRKRPWYVDAKRERKLVVTEPYVDISTKKIIISIGTPVYQQSNFVGAMFYDVELTQLAQLVNS
VNLFDAGYLFITTKDGVTIAHPNAENNGEKFSQFLPNVDLKEGTQRIELDGKYYLVKFAQVPSESWYIGAVVDESIAFAM
VDDLRHSSLIHHHHHH

0.1M Tris pH 8.5, 20% (w/v) PEG 8000, 0.2M magnesium chloride

Japan Society for the Promotion of Science

Ministry of Education, Culture, Sports, Science and Technology (Japan)

CHEMORECEPTOR, LIGAND COMPLEX, MCP-LIKE PROTEIN, PAS-LIKE DOMAIN, SIGNALING PROTEIN

VREEIESLVQDSLMEMVKGVKNTIESDLASKKGLAQSTTEILQLDPTNKAFAKSVLESPNLKGSFLAIGLGYESDATVVE
NDDGWEPNADYDPRKRPWYVDAKRERKLVVTEPYVDISTKKIIISIGTPVYQQSNFVGAMFYDVELTQLAQLVNSVNLFD
AGYLFITTKDGVTIAHPNAENNGEKFSQFLPNVDLKEGTQRIELDGKYYLVKFAQVPSESWYIGAVVDESIAFAMVDDLR
HSSLI