Crystal structure of RasADH F12 from Ralstonia.sp in complex with NADPH and A6O

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

Efficient reductive desymmetrization of bulky 1,3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase.

MYRLLNKTAVITGGNSGIGLATAKRFVAEGAYVFIVGRRRKELEQAAAEIGRNVTAVKADVTKLEDLDRLYAIVREQRGS
IDVLFANSGAVEQKTLEEITPEHYDRTFDVNVRGLIFTVQKALPLLRDGGSVILTSSVAGVLGLQAHDTYSAAKAAVRSL
ARTWTTELKGRSIRVNAVSPGAIDTPSLENNVSTQEEADELRAKAAAATPLGRVGRPEELAAAVLFLASDDSSYVAGIEL
FVDGGLTQV

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

OXIDOREDUCTASE, KRED, BIOCATALYST, HYDROLASE-INHIBITOR complex

MYRLLNKTAVITGGNSGIGLATAKRFVAEGAYVFIVGRRRKELEQAAAEIGRNVTAVKADVTKLEDLDRLYAIVREQRGS
IDVLFANSGAIEQKTLEEITPEHYDRTFDVNVRGLIFTVQKALPLLRDGGSVILTSSVAGVLGLQAHDTYSAAKAAVRSL
ARTWTTELKGRSIRVNAVSPGAIDTPIIENQVSTQEEADELRAKFAAATPLGRVGRPEELAAAVLFLASDDSSYVAGIEL
FVDGGLTQV