Crystal structure of Chloramphenicol-Metabolizaing Enzyme EstDL136-Chloramphenicol complex

Crystal structure of chloramphenicol-metabolizing enzyme EstDL136 from a metagenome.

To make EstDL136 crystal, internal residues from P37 to P39 were deleted

MPLNPHVEALLQMMAQMPAPDFSVANPAEIRAVFDNLAAPPQVARVENIAISLDGRDLDARLYVPEDADERPALMVYYHG
GGWVIGTLDTHDGTCRALAQKSGCAVLSIAYRLAPEYRYPAPAEDCYDALVWAKQNAATLGVDGDRLAVGGDAAGGNLAA
AVAIMARDRNGPALRHQLLIYPVTDNDFTLASYAENGGGEYYLSTDGMRWFWGHYLGDTAAENAPLAAVLNVADLSGLAP
ATVITAEYDPLRDEGIAYAKKLDAAGVPVDAATAPGMIHGFFSMFEAVPDSWEWIERGASNLKRDLALEHHHHHHHH

500mM Ammonium fluoride (pH6.5), 30% PEG 3350, 5% glyceol and 120mM TCEP.

Chloramphenicol, metagenome, hornome sensitive lipase, HSL, EstDL136, esterase, hydrolase

MPLNPHVEALLQMMAQMPAPDFSVANPAEIRAVFDNPMPLAAPPQVARVENIAISLDGRDLDARLYVPEDADERPALMVY
YHGGGWVIGTLDTHDGTCRALAQKSGCAVLSIAYRLAPEYRYPAPAEDCYDALVWAKQNAATLGVDGDRLAVGGDSAGGN
LAAAVAIMARDRNGPALRHQLLIYPVTDNDFTLASYAENGGGEYYLSTDGMRWFWGHYLGDTAAENAPLAAVLNVADLSG
LAPATVITAEYDPLRDEGIAYAKKLDAAGVPVDAATAPGMIHGFFSMFEAVPDSWEWIERGASNLKRDLA